AB109232

重组Anti-USP11抗体[EPR4346]

Name: USP11抗体[EPR4346] (ab109232)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109232
Price: 2529 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-USP11 antibody [EPR4346]

RabMAb
Recombinant
KO Validated
了解详情

(3 Reviews)

(27 Publications)

Rabbit Recombinant Monoclonal USP11 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 27 publications.

查看别名

UHX1, USP11, Ubiquitin carboxyl-terminal hydrolase 11, Deubiquitinating enzyme 11, Ubiquitin thioesterase 11, Ubiquitin-specific-processing protease 11

7 Images

Unknown

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232)

ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg HEK293 (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

Predicted band size: 110 kDa

false

Unknown

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232)

ab109232 (purified) at 1/300 immunoprecipitating USP11 in 10 μg Jurkat (Lanes 1 and 2, observed at 110 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (ab109232)

Predicted band size: 110 kDa

false

Lab

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution

Lane 1:

HEK293 lysate at 20 µg

Lane 2:

human fetal kidney lysate

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 110 kDa,115 kDa,132 kDa,27 kDa,53 kDa

Observed band size: 110 kDa,116 kDa,135 kDa,35 kDa

false

Unknown

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

All lanes:

Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Human testis cell lysate at 10 µg

Lane 4:

Huma fetal kidney cell lysate at 10 µg

Lane 5:

LnCaP cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Lab

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/1000 dilution

Lane 1:

Jurkat lysate at 20 µg

Lane 2:

human testis lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 110 kDa,28 kDa

Observed band size: 110 kDa

false

Lab

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : USP11 knockout HAP1 cell lysate (20 μg)
Lane 3 : LNCaP cell lysate (20 μg)
Lane 4 : HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109232 observed at 110 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109232 was shown to specifically react with USP11 when USP11 knockout samples were used. Wild-type and USP11 knockout samples were subjected to SDS-PAGE. ab109232 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-USP11 antibody [EPR4346] (ab109232)

Predicted band size: 110 kDa

false

Lab

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-USP11 antibody [EPR4346] (ab109232) at 1/5000 dilution

Lane 1:

mouse brain lysate at 20 µg

Lane 2:

rat brain lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1000 µg

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4346

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/300", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

USP11 also known as ubiquitin specific peptidase 11 is a deubiquitinating enzyme with a molecular mass of approximately 110 kDa. It removes ubiquitin molecules from target proteins regulating their stability and function. This enzyme is part of the USP family and exhibits strong expression in tissues like the testes brain and thyroid. The deubiquitination process is significant in maintaining protein homeostasis by recycling ubiquitins and controlling protein degradation.

Biological function summary

Ubiquitin-specific peptidases such as USP11 participate in diverse cellular functions including DNA repair signal transduction and cell cycle progression. USP11 often operates as part of a larger regulatory complex. It modulates DNA repair by interacting with proteins involved in the repair processes such as BRCA2. Through its deubiquitination activity USP11 significantly influences cellular response to DNA damage and helps to maintain genomic stability.

Pathways

USP11 plays a role in key mechanisms like the DNA damage response and NF-kB signaling pathways. It interacts with proteins involved in these pathways offering a layer of regulation. In the DNA damage response pathway USP11 associates with BRCA2 assisting in the repair of double-stranded breaks. In the NF-kB signaling pathway its activity can affect the degradation of IkB which allows the transcription factor NF-kB to enter the nucleus and promote gene expression.

Aberrations in USP11 function have been linked to cancer and neurodegenerative diseases. In cancer alterations in USP11 might contribute to tumorigenesis by affecting the DNA repair pathways especially through the BRCA2 protein interactions. Neurodegenerative diseases may also be connected to USP11 dysfunction due to its role in the degradation of proteins that accumulate in these conditions. The enzyme's interaction with various signaling proteins suggests a potential target for therapeutic interventions in these diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains (PubMed : 12084015, PubMed : 15314155, PubMed : 17897950, PubMed : 19874889, PubMed : 20233726, PubMed : 24724799, PubMed : 28992046). Inhibits the degradation of target proteins by the proteasome (PubMed : 12084015). Cleaves preferentially 'Lys-6' and 'Lys-63'-linked ubiquitin chains. Has lower activity with 'Lys-11' and 'Lys-33'-linked ubiquitin chains, and extremely low activity with 'Lys-27', 'Lys-29' and 'Lys-48'-linked ubiquitin chains (in vitro) (PubMed : 24724799). Plays a role in the regulation of pathways leading to NF-kappa-B activation (PubMed : 17897950, PubMed : 19874889). Plays a role in the regulation of DNA repair after double-stranded DNA breaks (PubMed : 15314155, PubMed : 20233726). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed : 20601937). Promotes cell proliferation by deubiquitinating phosphorylated E2F1 (PubMed : 28992046).

See full target information USP11

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文献 (27)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental medicine 25:267 PubMed40736712

2025

Mechanism of USP11 regulated SIRT3/ROS in drug resistance of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Pinwei Zhu,Hang Li,JinLi Ge,Zhencang Zhou,Dengke Chen,Qiang Li

Frontiers in molecular biosciences 12:1571593 PubMed40656894

2025

Inhibition of USP11 attenuates sepsis-associated acute kidney injury by downregulating TGFBR2/Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Wen Tang,Long Jiang,Daquan Zhang,Zhigao Wang,Rennan Guo,Jingjing Wang,Dong Xiao

Cell death & disease 15:873 PubMed39617751

2024

The USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Lu,Wanhui Wei,Mengting Li,Danyang Chen,Wenjie Li,Qian Hu,Shouquan Dong,Lan Liu,Qiu Zhao

Biochimica et biophysica acta. Molecular cell research 1872:119883 PubMed39603461

2024

Ubiquitin-specific peptidase 11 selectively interacts with and deubiquitination-dependently stabilizes diacylglycerol kinase δ to maintain cellular glucose uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Ebina,Yuri Miura,Fumio Sakane

EMBO molecular medicine 16:2856-2881 PubMed39394468

2024

Targeting USP11 regulation by a novel lithium-organic coordination compound improves neuropathologies and cognitive functions in Alzheimer transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Guo,Chuanbin Cai,Bingjie Zhang,Bo Tan,Qinmin Tang,Zhifeng Lei,Xiaolan Qi,Jiang Chen,Xiaojiang Zheng,Dan Zi,Song Li,Jun Tan

The Journal of biological chemistry 300:107768 PubMed39270819

2024

USP11 deubiquitinates E-cadherin and maintains the luminal fate of mammary tumor cells to suppress breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Qian,Feng Bai,Shiwen Zhang,Yuping Xu,Yuchan Wang,Shuping Yuan,Xiong Liu,Yaru Du,Bin Peng,Wei-Guo Zhu,Xingzhi Xu,Xin-Hai Pei

iScience 27:109708 PubMed38706856

2024

Positive and negative feedback regulation of the TGF-β1 explains two equilibrium states in skin aging.

Applications

Unspecified application

Species

Unspecified reactive species

Masatoshi Haga,Keita Iida,Mariko Okada

Cell death & disease 14:402 PubMed37414755

2023

USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Duan,Daoyuan Huang,Cheng Liu,Yangbo Lv,Lei Zhang,Fen Chang,Xiangyu Zeng,Li Li,Weiping Wang,Genze Shao

Frontiers in immunology 14:1134556 PubMed37205094

2023

Inhibition of the intracellular domain of Notch1 results in vascular endothelial cell dysfunction in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Tingyan Liu,Caiyan Zhang,Jiayun Ying,Yaodong Wang,Gangfeng Yan,Yufeng Zhou,Guoping Lu

Balkan medical journal 40:205-214 PubMed37000116

2023

Ubiquitin-specific protease 11 Aggravates Ischemia-reperfusion-induced Cardiomyocyte Pyroptosis and Injury by Promoting TRAF3 Deubiquitination

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei Zhang,Juledezi Hailati,Xiaoyun Ma,Hasidaer Midilibieke,Zhiqiang Liu

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-USP11抗体[EPR4346]

Anti-USP11 antibody [EPR4346]

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232)

Immunoprecipitation - Anti-USP11 antibody [EPR4346] (AB109232)

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

Western blot - Anti-USP11 antibody [EPR4346] (AB109232)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原