重组Anti-USF1抗体[EPR6430] (ab125020)
![Western blot - Anti-USF1 antibody [EPR6430] (ab125020)](https://www.abcam.cn/ps/products/125/ab125020/Images/ab125020-139148-anti-usf1-antibody-epr6430-western-blot.jpg "Western blot - Anti-USF1 antibody [EPR6430] (ab125020)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6430] to USF1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-USF1抗体[EPR6430]
参阅全部 USF1 一抗 -
描述
兔单克隆抗体[EPR6430] to USF1 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human USF1 aa 150-250. The exact sequence is proprietary.
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阳性对照
- K-562, Jurkat and HeLa whole cell lysate (ab150035); Jurkat cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 9.50 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6430 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125020于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
|
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| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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相关性
USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 7391 Human
- Entrez Gene: 22278 Mouse
- Entrez Gene: 83586 Rat
- Omim: 191523 Human
- SwissProt: P22415 Human
- SwissProt: Q61069 Mouse
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别名
- bHLHb11 antibody
- Class B basic helix-loop-helix protein 11 antibody
- FCHL 1 antibody
see all
图片
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Western blot - Anti-USF1 antibody [EPR6430] (ab125020)All lanes : Anti-USF1 antibody [EPR6430] (ab125020) at 1/10000 dilution
Lane 1 : K-562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Hela cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
![Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (ab125020)](https://www.abcam.cn/ps/products/125/ab125020/Images/ab125020-139147-anti-usf1-antibody-epr6430-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-USF1 antibody [EPR6430] (ab125020)Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab125020 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![OI-RD Scanning - Anti-USF1 antibody [EPR6430] (ab125020)](https://www.abcam.cn/ps/products/125/ab125020/Images/ab125020-139146-anti-usf1-antibody-epr6430.jpg)
OI-RD Scanning - Anti-USF1 antibody [EPR6430] (ab125020) -
![Anti-USF1 antibody [EPR6430] (ab125020)](https://www.abcam.cn/ps/products/125/ab125020/Images/ab125020-4-benefits-of-recombinant-antibodies.png)
Anti-USF1 antibody [EPR6430] (ab125020)
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab125020 被引用在 7 文献中.
- Peng JY et al. Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma. Adv Sci (Weinh) 10:e2204711 (2022). PubMed: 36307901
- Chen Q et al. miR-210-3p Promotes Lung Cancer Development and Progression by Modulating USF1 and PCGF3. Onco Targets Ther 14:3687-3700 (2021). PubMed: 34140779
- Li P et al. Inhibition of long noncoding RNA HIF1A-AS2 confers protection against atherosclerosis via ATF2 downregulation. J Adv Res 26:123-135 (2020). PubMed: 33133688
- Sun Q et al. LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway. Cell Prolif 52:e12687 (2019). PubMed: 31468594
- Guo J et al. Upstream stimulating factor 1 suppresses autophagy and hepatic lipid droplet catabolism by activating mTOR. FEBS Lett 592:2725-2738 (2018). PubMed: 30054905
- Song X et al. USF1 promotes the development of knee osteoarthritis by activating the NF-?B signaling pathway. Exp Ther Med 16:3518-3524 (2018). PubMed: 30233704
- Byun S et al. Src kinase is a direct target of apigenin against UVB-induced skin inflammation. Carcinogenesis 34:397-405 (2013). PubMed: 23161610