Anti-USF1抗体(ab180717)
Key features and details
- Rabbit polyclonal to USF1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-USF1抗体
参阅全部 USF1 一抗 -
描述
兔多克隆抗体to USF1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Cow
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免疫原
Recombinant full length protein corresponding to Human USF1 aa 1-310.
Sequence:MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG LEVVIKNDSN
Database link: P22415 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180717于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/200 - 1/500. Predicted molecular weight: 34 kDa.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/200 - 1/500. Predicted molecular weight: 34 kDa. |
靶标
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相关性
USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). Two transcript variants encoding distinct isoforms have been identified for this. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 7391 Human
- Entrez Gene: 22278 Mouse
- Entrez Gene: 83586 Rat
- Omim: 191523 Human
- SwissProt: Q6XBT4 Cow
- SwissProt: P22415 Human
- SwissProt: Q61069 Mouse
- SwissProt: O02818 Rabbit
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别名
- bHLHb11 antibody
- Class B basic helix-loop-helix protein 11 antibody
- FCHL 1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-USF1 antibody (ab180717)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab180717. Blue DAPI for nuclear staining.
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Western blot - Anti-USF1 antibody (ab180717)Anti-USF1 antibody (ab180717) at 1/500 dilution + K562 cell line extract
Predicted band size: 34 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab180717 被引用在 3 文献中.
- Wang Y et al. USF1 Transcriptionally Regulates UGT1A3 and Promotes Lung Adenocarcinoma Progression by Regulating Neurotrophin Signaling Pathway. Front Mol Biosci 9:758968 (2022). PubMed: 35155573
- Okamura M et al. Transcriptional Suppression of Diabetic Nephropathy with Novel Gene Silencer Pyrrole-Imidazole Polyamides Preventing USF1 Binding to the TGF-ß1 Promoter. Int J Mol Sci 22:N/A (2021). PubMed: 33947045
- Wang W et al. Upstream transcription factor 1 prompts malignancies of cervical cancer primarily by transcriptionally activating p65 expression. Exp Ther Med 16:4415-4422 (2018). PubMed: 30542391