Anti-Urokinase抗体(ab24121)
Key features and details
- Rabbit polyclonal to Urokinase
- Suitable for: IHC-Fr, ELISA, IHC-P, ICC/IF, Dot blot, RIA, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Urokinase抗体
参阅全部 Urokinase 一抗 -
描述
兔多克隆抗体to Urokinase -
宿主
Rabbit -
特异性
This antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa). This antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase. -
经测试应用
适用于: IHC-Fr, ELISA, IHC-P, ICC/IF, Dot blot, RIA, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length protein (Human). Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from human urine.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 8.00
Constituents: 0.16% Sodium phosphate, 0.38% Sodium borate, 0.87% Sodium chloride, 1% Dextran, 1% Mannitol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is subjected to absorption with immobilized total human serum proteins to remove non-specific antibodies. The affinity purified antibody is obtained by binding to immobilized native human urokinase, followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24121于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
|
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| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use at an assay dependent concentration.
|
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| Dot blot |
Use at an assay dependent concentration.
|
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| RIA |
Use at an assay dependent concentration.
|
|
| WB | (2) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
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Dot blot
Use at an assay dependent concentration. |
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RIA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
靶标
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功能
Specifically cleave the zymogen plasminogen to form the active enzyme plasmin. -
组织特异性
Expressed in the prostate gland and prostate cancers. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
翻译后修饰
Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5328 Human
- Omim: 191840 Human
- SwissProt: P00749 Human
- Unigene: 77274 Human
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别名
- ATF antibody
- ATF uPA antibody
- BDPLT5 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab24121 被引用在 18 文献中.
- Tan J et al. Proteomics analysis uncovers plasminogen activator PLAU as a target of the STING pathway for suppression of cancer cell migration and invasion. J Biol Chem 299:102779 (2023). PubMed: 36496076
- Hao L et al. CRISPR-Cas-amplified urinary biomarkers for multiplexed and portable cancer diagnostics. Nat Nanotechnol 18:798-807 (2023). PubMed: 37095220
- Zheng J et al. Deciphering intercellular signaling complexes by interaction-guided chemical proteomics. Nat Commun 14:4138 (2023). PubMed: 37438365
- Peng W et al. GLIPR1 Protects Against Cigarette Smoke-Induced Airway Inflammation via PLAU/EGFR Signaling. Int J Chron Obstruct Pulmon Dis 16:2817-2832 (2021). PubMed: 34675506
- Sundaram GM et al. HuR enhances FSTL1 transcript stability to promote invasion and metastasis of squamous cell carcinoma. Am J Cancer Res 11:4981-4993 (2021). PubMed: 34765305