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重组RabMAb

重组Anti-Urm1抗体[EPR4809] (ab110699)

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Western blot - Anti-Urm1 antibody [EPR4809] (ab110699)
  • Anti-Urm1 antibody [EPR4809] (ab110699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4809] to Urm1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Urm1抗体[EPR4809]
    参阅全部 Urm1 一抗

  • 描述

    兔单克隆抗体[EPR4809] to Urm1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human fetal liver, A549, Human fetal lung, HepG2, and DDDDK-tagged Urm1 recombinant protein.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR4809

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110699于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.

  • 通路

    tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

  • 序列相似性

    Belongs to the URM1 family.

  • 翻译后修饰

    C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 2900073H19Rik antibody
      • C9orf74 antibody
      • Chromosome 9 open reading frame 74 antibody
      • MGC2668 antibody
      • RGD1306599 antibody
      • RP11 339B21.4 antibody
      • RP23-443G7.1 antibody
      • Ubiquitin related modifier 1 antibody
      • Ubiquitin related modifier 1 homolog antibody
      • Ubiquitin-related modifier 1 homolog antibody
      • Urm 1 antibody
      • URM1 antibody
      • URM1_HUMAN antibody
      see all

    图片

    • Western blot - Anti-Urm1 antibody [EPR4809] (ab110699)
      Western blot - Anti-Urm1 antibody [EPR4809] (ab110699)
      All lanes : Anti-Urm1 antibody [EPR4809] (ab110699) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate at 10 µg
      Lane 2 : A549 lysate at 10 µg
      Lane 3 : Human fetal lung lysate at 10 µg
      Lane 4 : HepG2 lysate at 10 µg
      Lane 5 : DDDDK-tagged Urm1 recombinant protein at 0.01 µg

      Predicted band size: 11 kDa

    • Anti-Urm1 antibody [EPR4809] (ab110699)
      Anti-Urm1 antibody [EPR4809] (ab110699)

    文献 (0)

    发表研究结果有使用 ab110699？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab110699 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    I saw on your website that there may be a discount for testing product
    number ab110699 for reactivity with S. pombe. We would like to try
    this antibody and would be willing to test it against S. pombe as
    well.

    Read More
    Answer

    Thank you for your interest in the testing discount program. We may be able to offer the testing discount that you saw mentioned on the Scientific support section of the online datasheet. It will depend on the nature of your assay: western blotting, immunocytochemistry, ELISA, etc. Can you please tell me what kind of assay you anticipate using? We are currently only recommending it for western blotting.

    Read More

    Question

    Anti-Urm1 antibody, reacting with S. pombe

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    Answer

    Thank you for your enquiry. I was passed your details by Aris in our marketing department. We have two antibodies that may be of interest: ab4755  Anti-Urm1 antibody      ab110699  Anti-Urm1 antibody [EPR4809]   To our knowledge, these antibodies have not been tested in S. pombe.  Therefore, I can offer a discount off a future purchase if you buy one of these products now, test it in S. pombe and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.   If you are interested in this offer, please follow these steps: 1.   Reply to this e-mail to let me know that you would like to proceed and test one of the products in S. pombe. I will then send a discount code. This code must be issued before purchasing  the product so please wait for my reply before ordering. 2.   Purchase the antibody either by phone, fax, or online (www.abcam.com). 3.   Test it in S. pombe 4.   Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).  To find out how to submit an Abreview, please visit:  https://www.abcam.com/abreviews. 5.   After the review is submitted to us, the discount code becomes active.  Simply place your new order by phone, fax, or on the web and mention the discount code.   The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.   We are always pleased to obtain feedback about our products and any information is greatly appreciated!  Even if the product turns out to be unsuitable, you will still receive the discount on your next purchase after your Abreview has been submitted.   Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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