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重组RabMAb

重组Anti-Uridine Phosphorylase 1抗体[EPR7680] (ab128854)

Reviews (1) Submit a question References (2)
Western blot - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
  • OI-RD Scanning - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
  • Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7680] to Uridine Phosphorylase 1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Uridine Phosphorylase 1抗体[EPR7680]
    参阅全部 Uridine Phosphorylase 1 一抗

  • 描述

    兔单克隆抗体[EPR7680] to Uridine Phosphorylase 1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human Uridine Phosphorylase 1. The exact sequence is proprietary.

  • 阳性对照

    • 293T cell lysate; HeLa cell lysate; HCT116 cell lysate; Human fetal kidney tissue lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab128854于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 34 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 34 kDa).
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

  • 通路

    Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.

  • 序列相似性

    Belongs to the PNP/UDP phosphorylase family.
  • Information by UniProt

  • 数据库链接

    • 别名

      • UDRPASE antibody
      • UP antibody
      • UPase 1 antibody
      • UPASE antibody
      • UPP antibody
      • Upp1 antibody
      • UPP1_HUMAN antibody
      • UrdPase 1 antibody
      • Uridine phosphorylase 1 antibody
      see all

    图片

    • Western blot - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
      Western blot - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
      All lanes : Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HCT116 cell lysate
      Lane 4 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 34 kDa

    • OI-RD Scanning - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
      OI-RD Scanning - Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)
      Anti-Uridine Phosphorylase 1 antibody [EPR7680] (ab128854)

    文献 (2)

    发表研究结果有使用 ab128854？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab128854 被引用在 2 文献中.

    • Wang X  et al. UPP1 Promotes Lung Adenocarcinoma Progression through Epigenetic Regulation of Glycolysis. Aging Dis 13:1488-1503 (2022). PubMed: 36186123
    • Wu Z  et al. Identification of crucial genes of pyrimidine metabolism as biomarkers for gastric cancer prognosis. Cancer Cell Int 21:668 (2021). PubMed: 34906153

    客户评价及客户问答

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    Western blot abreview for Anti-Uridine Phosphorylase 1 antibody [EPR7680]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lung cancer)
    Loading amount
    25 µg
    Specification
    Lung cancer
    Treatment
    shUpp1
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 08 2013

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