Anti-ureB抗体(ab127916)
Key features and details
- Rabbit polyclonal to ureB
- Suitable for: WB, IHC-P
- Reacts with: Helicobacter pylori
- Isotype: IgG
概述
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产品名称
Anti-ureB抗体
参阅全部 ureB 一抗 -
描述
兔多克隆抗体to ureB -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Helicobacter pylori -
免疫原
Recombinant fragment corresponding to ureB aa 341-398.
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阳性对照
- ureB whole cell lysate; Mice Gastric pylori.
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常规说明
Keep as concentrated solution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab127916于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000. Predicted molecular weight: 62 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM
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| 说明 |
|---|
|
WB
1/50000. Predicted molecular weight: 62 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM |
靶标
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功能
Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach. -
通路
Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. -
序列相似性
Belongs to the urease family.
Contains 1 urease domain. -
翻译后修饰
Carbamylation allows a single lysine to coordinate two nickel ions. -
细胞定位
Cytoplasm. Also associates with the outer membrane upon autolysis of neighboring bacteria. - Information by UniProt
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别名
- HP_0072 antibody
- hpuB antibody
- URE1_HELPY antibody
see all
图片
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Western blot - Anti-ureB antibody (ab127916)All lanes : Anti-ureB antibody (ab127916) at 1/50000 dilution
Lane 1 : ureB at 0.5 µg
Lane 2 : ureB at 1 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ureB antibody (ab127916)ab127916, at 1/500 dilution, staining ureB in paraffin-embedded Mice Gastric pylori, by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab127916 被引用在 4 文献中.
- Liu M et al. Oral immunization of mice with a multivalent therapeutic subunit vaccine protects against Helicobacter pylori infection. Vaccine 38:3031-3041 (2020). PubMed: 32139315
- Valenzuela-Valderrama M et al. The Helicobacter pylori Urease Virulence Factor Is Required for the Induction of Hypoxia-Induced Factor-1a in Gastric Cells. Cancers (Basel) 11:N/A (2019). PubMed: 31185594
- Ohishi T et al. Monotherapy with a novel intervenolin derivative, AS-1934, is an effective treatment for Helicobacter pylori infection. Helicobacter 23:e12470 (2018). WB . PubMed: 29488678
- Li N et al. Helicobacter pylori CagA promotes epithelial mesenchymal transition in gastric carcinogenesis via triggering oncogenic YAP pathway. J Exp Clin Cancer Res 37:280 (2018). PubMed: 30466467