Anti-UPF3B/RENT3B抗体(ab134566)
Key features and details
- Rabbit polyclonal to UPF3B/RENT3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UPF3B/RENT3B抗体
参阅全部 UPF3B/RENT3B 一抗 -
描述
兔多克隆抗体to UPF3B/RENT3B -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Bat -
免疫原
Synthetic peptide corresponding to Human UPF3B/RENT3B aa 300-400.
Database link: Q9BZI7 -
阳性对照
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134566于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core. -
组织特异性
Expressed in testis, uterus, prostate, heart, muscle, brain, spinal cord and placenta. -
疾病相关
Mental retardation, X-linked, syndromic, 14 -
序列相似性
Belongs to the RENT3 family. -
细胞定位
Nucleus. Cytoplasm. Shuttling between the nucleus and the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 65109 Human
- Omim: 300298 Human
- SwissProt: Q9BZI7 Human
- Unigene: 103832 Human
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别名
- hUpf3B antibody
- hUpf3p X antibody
- hUpf3p-X antibody
see all
图片
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All lanes : Anti-UPF3B/RENT3B antibody (ab134566) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 10 seconds -
Detection of UPF3B/RENT3B by Western Blot of Immunprecipitate.
Immunoprecipitation of HeLa whole cell lysate using ab134566 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection of UPF3B/RENT3B with ab134566 at 1µg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab134566 被引用在 3 文献中.
- Cho H et al. AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay. Mol Cell 82:2779-2796.e10 (2022). PubMed: 35675814
- Palma M et al. A role for AKT1 in nonsense-mediated mRNA decay. Nucleic Acids Res 49:11022-11037 (2021). PubMed: 34634811
- Fontaine KA et al. The Cellular NMD Pathway Restricts Zika Virus Infection and Is Targeted by the Viral Capsid Protein. MBio 9:N/A (2018). PubMed: 30401782