Anti-UNG抗体(ab47680)
Key features and details
- Rabbit polyclonal to UNG
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-UNG抗体
参阅全部 UNG 一抗 -
描述
兔多克隆抗体to UNG -
宿主
Rabbit -
特异性
This antibody is specific to UNG1. It will not cross react with UNG2. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
A 13 amino acid peptide from near the amino terminus of human UNG1
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阳性对照
- C2C12 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47680于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. -
组织特异性
Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells. -
疾病相关
Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 syndrome (HIGM5) [MIM:608106]. Hyper-IgM syndrome is a condition characterized by normal or increased serum IgM concentrations associated with low or absent serum IgG, IgA, and IgE concentrations. HIGM5 is associated with profound impairment in immunoglobulin (Ig) class-switch recombination (CSR) at a DNA precleavage step. -
序列相似性
Belongs to the uracil-DNA glycosylase family. -
翻译后修饰
Isoform 1 is processed by cleavage of a transit peptide. -
细胞定位
Mitochondrion and Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7374 Human
- Entrez Gene: 22256 Mouse
- Omim: 191525 Human
- SwissProt: P13051 Human
- SwissProt: P97931 Mouse
- Unigene: 191334 Human
- Unigene: 1393 Mouse
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别名
- DGU antibody
- DKFZp781L1143 antibody
- HIGM 4 antibody
see all
图片
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Lane 1 : Anti-UNG antibody (ab47680) at 0.5 µg/ml
Lane 2 : Anti-UNG antibody (ab47680) at 1 µg/ml
Lane 3 : Anti-UNG antibody (ab47680) at 2 µg/ml
All lanes : C2C12 lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Anti rabbit IgG secondary antibodies can be used. -
ab37170 at 2 µg/ml staining UNG in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunofluorescence of UNG1 in Human Heart cells using ab47680 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab47680 被引用在 1 文献中.
- Ferrando B et al. DNA repair in plant mitochondria - a complete base excision repair pathway in potato tuber mitochondria. Physiol Plant 166:494-512 (2019). PubMed: 30035320