重组Anti-ULK3抗体[EPR4888]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULK3 antibody [EPR4888] (AB124947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ULK3 with purified ab124947 at 1/50 dilution (7.84 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ULK3 antibody [EPR4888] (AB124947)

ab124947 (unpurified), at a 1/50 dilution, staining ULK3 in paraffin embedded Human bladder carcinoma tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ULK3 antibody [EPR4888] (AB124947)

Intracellular Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells, labeling ULK3 with Purified ab124947 at 1/40 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ULK3 antibody [EPR4888] (AB124947)

Immunocytochemistry/Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling ULK3 with Purified ab124947 at 1 : 50 dilution (7.8 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Lab

Western blot - Anti-ULK3 antibody [EPR4888] (AB124947)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : ULK3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Hek293 whole cell lysate (20 μg)
Lanes 1 - 3 : Merged signal (red and green). Green - ab124947 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab124947 (unpurified) was shown to specifically react with ULK3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ULK3 knockout samples were examined. Wild-type and ULK3 knockout samples were subjected to SDS-PAGE. ab124947 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ULK3 antibody [EPR4888] (ab124947)

Predicted band size: 102 kDa,121 kDa,18 kDa,53 kDa

Observed band size: 150 kDa

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• WB

Unknown

Western blot - Anti-ULK3 antibody [EPR4888] (AB124947)

Blocking/Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human testis lysate at 20 µg

Lane 3:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

false

• WB

Lab

Western blot - Anti-ULK3 antibody [EPR4888] (AB124947)

Lanes 1-4 : Merged signal (red and green). Green - ab124947 observed at 55 kDa. Red - loading control ab8245 observed at 36 kDa.

ab124947 Anti-ULK3 antibody [EPR4888] was shown to specifically react with ULK3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266152 (knockout cell lysate ab258271) was used. Wild-type and ULK3 knockout samples were subjected to SDS-PAGE. ab124947 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ULK3 antibody [EPR4888] (ab124947) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

ULK3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ULK3 knockout HEK-293T cell line (<a href='/products/cell-lines/human-ulk3-knockout-hek-293t-cell-line-ab266152'>ab266152</a>)

Lane 3:

HAP1 whole cell lyate at 20 µg

Lane 4:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 53 kDa

Observed band size: 55 kDa

false