AB281113

重组Anti-ULBP3抗体[EPR22321-99] - BSA and Azide free (Detector)

Anti-ULBP3 antibody [EPR22321-99] - BSA and Azide free (Detector)

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal ULBP3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

查看别名

N2DL3, RAET1N, ULBP3, UL16-binding protein 3, ALCAN-gamma, NKG2D ligand 3, Retinoic acid early transcript 1N, N2DL-3, NKG2DL3

2 Images

Lab

Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] - BSA and Azide free (Detector) (AB281113)

This data was developed using ab300102, the same antibody clone in a different buffer formulation.

ULBP3 was immunoprecipitated from 0.35 mg SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab300102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300102 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ULBP3 antibody [EPR22321-99] (<a href='/products/primary-antibodies/ulbp3-antibody-epr22321-99-ab300102'>ab300102</a>) at 1/30 dilution

Lane 1:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/ulbp3-antibody-epr22321-99-ab300102'>ab300102</a> in SW480 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 27 kDa

false

Exposure time: 93s

Sandwich ELISA - Anti-ULBP3 antibody [EPR22321-99] - BSA and Azide free (Detector) (AB281113)

sELISA

Supplier Data

Sandwich ELISA - Anti-ULBP3 antibody [EPR22321-99] - BSA and Azide free (Detector) (AB281113)

Example of Human ULBP3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

不同偶联物与剂型 (2)

Carrier free

Anti-ULBP3 antibody [EPR22321-99] (BSA and Azide free)
Unconjugated

Anti-ULBP3 antibody [EPR22321-99]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22321-99

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab281113 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ULBP3. The recommended pair for sandwich ELISA is:
Capture: ab281263, Human ULBP3 Capture Antibody (unconjugated)
Detector: ab281113, Human ULBP3 Detector Antibody (unconjugated)
The reference range value is 31 - 2000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ULBP3 also known as UL16 binding protein 3 plays a significant role in immune surveillance. The protein is a member of the NKG2D ligand family and has a mass of approximately 30 kDa. ULBP3 is expressed on the cell surface of many human tissues predominantly in stressed transformed or infected cells where it becomes more prominent. The expression of ULBP3 signals to immune cells especially natural killer (NK) cells and some subsets of T-cells acting as an essential point of recognition and interaction.

Biological function summary

ULBP3 interacts with the activating receptor NKG2D to initiate immune responses against tumor cells or pathogen-infected cells. The binding of ULBP3 to NKG2D forms a part of the recognition process leading to the activation of NK cells and cytotoxic T cells enhancing their ability to destroy target cells. ULBP3 operates independently and not as part of a larger ligand complex making its interactions directly important for regulating immune function across different environments and conditions aiding in the maintenance of cellular homeostasis.

Pathways

ULBP3 participates integrally in immune signaling pathways like the NKG2D receptor-mediated pathway. Within this pathway its role in signaling enhances NK cell cytotoxicity and cytokine production. ULBP3 is related to other NKG2D ligands like MICA MICB and other ULBP molecules through this immune recognition pathway collectively contributing to immune defense mechanisms. These pathways form a critical element of the broader immune surveillance mechanism in the human body.

ULBP3 has associations with various cancers and infectious diseases. Overexpression of ULBP3 has been observed in certain types of cancers such as lymphoma where it promotes recognition by the NK cells. Another area of interest is its role in viral infections where pathogens may modulate ULBP3 expression to evade the immune system. ULBP3's interaction with proteins like NKG2D is vital in modulating immune responses during these diseases highlighting its potential both as a target for therapeutic intervention and a biomarker for disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.

See full target information ULBP3

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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重组Anti-ULBP3抗体[EPR22321-99] - BSA and Azide free (Detector)