重组Anti-ULBP2抗体[EPR22303-124] - BSA and Azide free (ab275034)
![Western blot - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)](https://www.abcam.cn/ps/products/275/ab275034/Images/ab275034-403296-antiulbp2-antibody-epr22303-124-western-blot-human.jpg "Western blot - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22303-124] to ULBP2 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ULBP2抗体[EPR22303-124] - BSA and Azide free
参阅全部 ULBP2 一抗 -
描述
兔单克隆抗体[EPR22303-124] to ULBP2 - BSA and Azide free -
宿主
Rabbit -
特异性
We have preliminary internal testing data to indicate this antibody weakly cross reacts with recombinant human ULBP5 protein in WB. Please contact us for more information.
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经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: BxPC-3, MOLT-4, HeLa, HCT116 and U-87 MG whole cell lysates. IP: BxPC-3 whole cell lysate.
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常规说明
ab275034 is the carrier-free version of ab275023.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22303-124 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab275034于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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| IP |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis-Golgi apparatus so that it does not reach the cell surface. -
组织特异性
Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues. -
序列相似性
Belongs to the MHC class I family. -
细胞定位
Cell membrane. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 80328 Human
- Omim: 605698 Human
- SwissProt: Q9BZM5 Human
- Unigene: 656778 Human
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别名
- ALCAN alpha antibody
- ALCAN-alpha antibody
- N2DL 2 antibody
see all
图片
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Western blot - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)All lanes : Anti-ULBP2 antibody [EPR22303-124] (ab275023) at 1/1000 dilution
Lane 1 : BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 5 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 6 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDaThis data was developed using ab275023, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds Lane 2-7: 3 minutes.
Negative control: Raji, Daudi (PMID: 12414645).
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![Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)](https://www.abcam.cn/ps/products/275/ab275034/Images/ab275034-1-anti-ulbp2-antibody-epr22303124-immunoprecipitation-bxpc3.jpg)
Immunoprecipitation - Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)This data was developed using ab275023, the same antibody clone in a different buffer formulation.
ULBP2 was immunoprecipitated from 0.35 mg BxPC-3 (human pancreas adenocarcinoma epithelial cell), whole cell lysate with ab275023 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275023 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: BxPC-3 whole cell lysate 10 µg
Lane 2: ab275023 IP in BxPC-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275023 in BxPC-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds.
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![Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)](https://www.abcam.cn/ps/products/275/ab275034/Images/ab275034-3-benefits-of-recombinant-antibodies.png)
Anti-ULBP2 antibody [EPR22303-124] - BSA and Azide free (ab275034)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab275034 尚未被引用在任何文献中。
