Anti-UHRF1抗体(ab126243)
Key features and details
- Rabbit polyclonal to UHRF1
- Suitable for: IHC-P, ChIP, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UHRF1抗体
参阅全部 UHRF1 一抗 -
描述
兔多克隆抗体to UHRF1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ChIP, WB, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 503 and 733 of Human UHRF1 (AAI13876)
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阳性对照
- ChIP: HCT 116 chromatin extract. WB: HCT116 whole cell lysate. IHC-P: HBL435 xenograft tissue. IF: A549 and HeLa cells. IP: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| ChIP |
Use at an assay dependent concentration.
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| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| 说明 |
|---|
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IHC-P
1/100 - 1/1000. |
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ChIP
Use at an assay dependent concentration. |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
靶标
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功能
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. -
组织特异性
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain. -
发展阶段
Expressed in fetal thymus, liver and kidney. -
结构域
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3. -
翻译后修饰
Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29128 Human
- Omim: 607990 Human
- SwissProt: Q96T88 Human
- Unigene: 108106 Human
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别名
- Ac2-121 antibody
- AL022808 antibody
- E3 ubiquitin-protein ligase UHRF1 antibody
see all
图片
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ChIP - Anti-UHRF1 antibody (ab126243)
Cross-linked ChIP was performed with HCT 116 (human colorectal carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab126243. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.
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Western blot - Anti-UHRF1 antibody (ab126243)Anti-UHRF1 antibody (ab126243) at 1/1000 dilution + HCT116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 51 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody (ab126243)Paraffin-embedded HBL435 xenograft tissue stained UHRF1 using ab126243 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab126243)Immunofluorescence analysis of 4% paraformaldehyde fixed (RT for 15 min) A549 (human lung carcinoma cell line) whole cell labelling UHRF1 in the nucleus (green) at 1/250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/1000 dilution.
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Immunoprecipitation - Anti-UHRF1 antibody (ab126243)IP Sample: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate.
1 - 40 μg HeLa whole cell lysate.
2 - Control with 2 μg of preimmune rabbit IgG.
3 - Immunoprecipitation of UHRF1 protein by 2 μg of ab126243.
5% SDS-PAGE
The immunoprecipitated UHRF1 protein was detected by ab126243 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody (ab126243)Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling UHRF1 using ab126243 (green) at 1/500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at 1/2000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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