Anti-UHRF1抗体[3A11] (ab57083)
![Western blot - Anti-UHRF1 antibody [3A11] (ab57083)](https://www.abcam.cn/ps/products/57/ab57083/Images/ab57083-70608-anti-uhrf1-antibody-western-blot.jpg "Western blot - Anti-UHRF1 antibody [3A11] (ab57083)")
Key features and details
- Mouse monoclonal [3A11] to UHRF1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-UHRF1抗体[3A11]
参阅全部 UHRF1 一抗 -
描述
小鼠单克隆抗体[3A11] to UHRF1 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: WNEVLASLKD RPASGSPFQL FLSKVEETFQ CICCQELVFR PITTVCQHNV CKDCLDRSFR AQVFSCPACR YDLGRSYAMQ VNQPLQTVLN QLFPGYGNGR , corresponding to amino acids 694-794 of Human UHRF1
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常规说明
This product was changed from ascites to tissue culture supernatant on 25/2/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
3A11 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab57083于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 90 kDa.
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| IHC-P | (3) |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 90 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability. -
组织特异性
Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 PHD-type zinc finger.
Contains 2 RING-type zinc fingers.
Contains 1 ubiquitin-like domain.
Contains 1 YDG domain. -
发展阶段
Expressed in fetal thymus, liver and kidney. -
结构域
The RING finger is required for ubiquitin ligase activity.
The YDG domain mediates the interaction with histone H3. -
翻译后修饰
Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.
Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29128 Human
- Omim: 607990 Human
- SwissProt: Q96T88 Human
- Unigene: 108106 Human
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别名
- Ac2-121 antibody
- AL022808 antibody
- E3 ubiquitin-protein ligase UHRF1 antibody
see all
图片
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Western blot - Anti-UHRF1 antibody [3A11] (ab57083)
UHRF1 antibody (ab57083) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [3A11] (ab57083)](https://www.abcam.cn/ps/products/57/ab57083/Images/ab57083-70609-anti-uhrf1-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UHRF1 antibody [3A11] (ab57083)UHRF1 antibody (ab57083) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [3A11] (ab57083)](https://www.abcam.cn/ps/products/57/ab57083/Images/ab57083-70607-anti-uhrf1-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UHRF1 antibody [3A11] (ab57083)ICC/IF image of ab57083 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57083, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-UHRF1 antibody [3A11] (ab57083)](https://www.abcam.cn/ps/products/57/ab57083/Images/ab57083-70606-anti-uhrf1-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-UHRF1 antibody [3A11] (ab57083)Overlay histogram showing MCF7 cells stained with ab57083 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57083, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab57083 被引用在 19 文献中.
- Bhandari SK et al. Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1. Sci Rep 13:4363 (2023). PubMed: 36928068
- Wu S et al. Interfering with the Expression of Ubiquitin-Like with PHD and Ring Finger Domains 1 Can Inhibit the Invasion of Human Renal Cell Carcinoma. Comput Math Methods Med 2022:6971131 (2022). PubMed: 37671388
- Yao J et al. UHRF1 regulates the transcriptional repressor HBP1 through MIF in T acute lymphoblastic leukemia. Oncol Rep 46:N/A (2021). PubMed: 34013373
- Barcena-Varela M et al. Epigenetic mechanisms and metabolic reprogramming in fibrogenesis: dual targeting of G9a and DNMT1 for the inhibition of liver fibrosis. Gut 70:388-400 (2021). PubMed: 32327527
- Zhang Q et al. UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells. Cell Cycle 17:300-308 (2018). PubMed: 29157076
