Anti-UGT抗体[7H2AF11] (ab129729)
Key features and details
- Mouse monoclonal [7H2AF11] to UGT
- Suitable for: IHC-P, IP, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-UGT抗体[7H2AF11] -
描述
小鼠单克隆抗体[7H2AF11] to UGT -
宿主
Mouse -
经测试应用
适用于: IHC-P, IP, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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阳性对照
- Human liver homogenate lysate; Hela cells This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
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纯化说明
Purity is near homogeneity as judged by SDS-PAGE. ab129729 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
7H2AF11 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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细胞定位
Microsome membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein -
数据库链接
- Entrez Gene: 54490 Human
- Entrez Gene: 54600 Human
- Entrez Gene: 54658 Human
- Entrez Gene: 7363 Human
- Omim: 191740 Human
- Omim: 600067 Human
- Omim: 606434 Human
- Omim: 606497 Human
see all -
别名
- Bilirubin-specific UDPGT isozyme 1 antibody
- Hyodeoxycholic acid-specific UDPGT antibody
- UDP-glucuronosyltransferase 1-1 antibody
see all
图片
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Overlay histogram showing HepG2 cells stained with ab129729 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129729, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse Alexa Fluor® 488 (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UGT antibody [7H2AF11] (ab129729)
IHC image of UGT staining in Human Normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129729, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunoprecipitation image of ab129729. UGT antibody immunocaptured 57 kDa UGT from Human liver homogenate (HLH). Gel stained with Coomassie brilliant blue G.
Lane 1: Ladder Lane 2: Human liver homogenate lysate -
Immunocytochemistry image of ab129729 stained Hela cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab129729 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates to the endoplasmic reticulum.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab129729 被引用在 1 文献中.
- Obst JK et al. Revealing Metabolic Liabilities of Ralaniten To Enhance Novel Androgen Receptor Targeted Therapies. ACS Pharmacol Transl Sci 2:453-467 (2019). PubMed: 32259077