Anti-UCP3抗体[mAbcam61299] (ab61299)
![Western blot - Anti-UCP3 antibody [mAbcam61299] (ab61299)](https://www.abcam.cn/ps/products/61/ab61299/Images/ab61299-74783-anti-ucp3-antibody-mabcam61299-western-blot.jpg "Western blot - Anti-UCP3 antibody [mAbcam61299] (ab61299)")
Key features and details
- Mouse monoclonal [mAbcam61299] to UCP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-UCP3抗体[mAbcam61299]
参阅全部 UCP3 一抗 -
描述
小鼠单克隆抗体[mAbcam61299] to UCP3 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Pig, Macaque monkey
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免疫原
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阳性对照
- This antibody gave a positive signal in human skeletal muscle tissue lysate.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Concentrated Culture Supernatant -
纯化说明
Tissue culture supernatant was cross-flow concentrated and buffer exchanged to PBS. -
克隆
单克隆 -
克隆编号
mAbcam61299 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. As a result, energy is dissipated in the form of heat. May play a role in the modulation of tissue respiratory control. Participates in thermogenesis and energy balance. -
组织特异性
Only in skeletal muscle and heart. Is more expressed in glycolytic than in oxidative skeletal muscles. -
疾病相关
Defects in UCP3 may be involved in obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
序列相似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7352 Human
- Entrez Gene: 397116 Pig
- Omim: 602044 Human
- SwissProt: P55916 Human
- SwissProt: O97649 Pig
- Unigene: 101337 Human
- Unigene: 621879 Human
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形式
UCP3 is preferentially expressed in muscle -
别名
- Mitochondrial uncoupling protein 3 antibody
- SLC25A9 antibody
- Solute carrier family 25 member 9 antibody
see all
图片
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Western blot - Anti-UCP3 antibody [mAbcam61299] (ab61299)Anti-UCP3 antibody [mAbcam61299] (ab61299) at 10 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgM - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
数据表及文件
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Datasheet download
文献 (0)
ab61299 尚未被引用在任何文献中。
