Anti-UCP1抗体(ab23841)
Key features and details
- Rabbit polyclonal to UCP1
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-UCP1抗体
参阅全部 UCP1 一抗 -
描述
兔多克隆抗体to UCP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Hamster, Dog, Human, Chimpanzee -
免疫原
Synthetic peptide corresponding to Human UCP1 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24282) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23841于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
靶标
-
功能
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. -
组织特异性
Brown adipose tissue. -
序列相似性
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 7350 Human
- Entrez Gene: 22227 Mouse
- Entrez Gene: 24860 Rat
- Omim: 113730 Human
- SwissProt: P25874 Human
- SwissProt: P12242 Mouse
- SwissProt: P04633 Rat
- Unigene: 249211 Human
see all -
形式
UCP1 is preferentially expressed in brown adipose tissue -
别名
- mitochondrial brown fat uncoupling protein antibody
- Mitochondrial brown fat uncoupling protein 1 antibody
- SLC25A7 antibody
see all
图片
-
All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml
Lane 1 : Mouse brown adipose cell lysate
Lane 2 : Mouse white adipose cell lysate
Lane 3 : Rat brown adipose cell lysate
Lane 4 : Rat white adipose cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30-33 kDa why is the actual band size different from the predicted?Western blot: Anti-UCP1 antibody (ab23841) staining at 1 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab23841 was shown to bind specifically to UCP1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
All lanes : Anti-UCP1 antibody (ab23841) at 1 µg/ml
Lane 1 : Adult Mouse Brown Adipose Tissue Lysate
Lane 2 : Adult Rat Brown Adipose Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
数据表及文件
-
SDS download
-
Datasheet download
文献 (151)
ab23841 被引用在 151 文献中.
- Da Eira D et al. An obesogenic diet impairs uncoupled substrate oxidation and promotes whitening of the brown adipose tissue in rats. J Physiol 601:69-82 (2023). PubMed: 36419345
- Chen W et al. Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy. BMC Biol 21:27 (2023). PubMed: 36750818
- Lee HJ et al. Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes. Nat Commun 14:2754 (2023). PubMed: 37179330
- Dieckmann S et al. Susceptibility to diet-induced obesity at thermoneutral conditions is independent of UCP1. Am J Physiol Endocrinol Metab 322:E85-E100 (2022). PubMed: 34927460
- Xing C et al. Lysyl oxidase inhibition enhances browning of white adipose tissue and adaptive thermogenesis. Genes Dis 9:140-150 (2022). PubMed: 35005114