AB249915

重组Anti-UBLCP1抗体[EPR11923] - BSA and Azide free

Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal UBLCP1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

Ubiquitin-like domain-containing CTD phosphatase 1, Nuclear proteasome inhibitor UBLCP1, UBLCP1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free (AB249915)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free (AB249915)

This data was developed using ab176340, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.

Supplier Data

Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free (AB249915)

This data was developed using ab176340, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (<a href='/products/primary-antibodies/ublcp1-antibody-epr11923-ab176340'>ab176340</a>)

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-UBLCP1 antibody [EPR11923] - BSA and Azide free (AB249915)

This data was developed using ab176340, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-UBLCP1 antibody [EPR11923] (<a href='/products/primary-antibodies/ublcp1-antibody-epr11923-ab176340'>ab176340</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Predicted band size: 37 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-UBLCP1 antibody [EPR11923]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11923

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab249915 is the carrier-free version of ab176340.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

UBLCP1 also known as Ubiquitin-Like Domain Containing CTD Phosphatase 1 is a phosphatase that primarily functions in dephosphorylation of substrate proteins. This protein has a molecular mass of approximately 44 kDa and is expressed in various human tissues but shows higher levels in the brain and skeletal muscle. UBLCP1 contains ubiquitin-like domains which indicate its potential interactions with other molecules containing ubiquitin or ubiquitin-like proteins influencing its mechanical roles inside cells.

Biological function summary

UBLCP1 plays a significant role in regulating cellular processes by removing phosphate groups from substrates which can affect protein activity cellular localization or interaction with other proteins. It does not work alone but associates with other molecules to form complexes that allow it to control different cellular processes effectively. One such interaction is with the RNA polymerase II where UBLCP1 may regulate transcription by dephosphorylating the C-terminal domain of RNA polymerase II.

Pathways

Several studies have linked UBLCP1 to key signaling pathways that control cell cycle and transcription regulation. It interacts with pathways involving RNA polymerase II influencing its activity during transcription. Additionally UBLCP1 shares interactions with components of the TGF-beta pathway though details of this relationship require further study. This connection could suggest a role in controlling growth and differentiation signals in cells.

Researchers associate UBLCP1 with neurodegenerative diseases including Alzheimer's disease given its higher expression in brain tissues and involvement in transcriptional regulation. Perturbations in UBLCP1's activity could affect proteins related to synaptic function and memory. Moreover UBLCP1 interaction with proteins like RNA polymerase II indicates that it may influence diseases where transcription is disrupted such as certain cancers. Further understanding of its mechanistic role could provide insights into novel therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity (PubMed : 21949367, PubMed : 28539385). Recruited to the 19S regulatory particle of the 26S proteasome through its interaction with 19S component PSMD2/RPN1 (PubMed : 28539385). Once recruited, dephosphorylates 19S component PSMC2/RPT1 which impairs PSMC2 ATPase activity and disrupts 26S proteasome assembly (PubMed : 28539385). Has also been reported to stimulate the proteolytic activity of the 26S proteasome (PubMed : 32071216).

See full target information UBLCP1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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重组Anti-UBLCP1抗体[EPR11923] - BSA and Azide free