重组Anti-Ubiquitin (linkage-specific K63)抗体[EPR8590-448] (ab179434)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Ubiquitin (linkage-specific K63)抗体[EPR8590-448]
参阅全部 Ubiquitin 一抗 -
描述
兔单克隆抗体[EPR8590-448] to Ubiquitin (linkage-specific K63) -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: K63-linked-Ub2-7 recombinant protein, HEK-293 and HeLa cell lysates, and Mouse and rat brain lysate. IHC-P: Human kidney carcinoma and tonsil tissue. Flow Cyt (intra): HeLa cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8590-448 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab192489)
- Alexa Fluor® 488 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab192539)
- Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - Low endotoxin, Azide free (ab220794)
- Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] - BSA and Azide free (ab271929)
- Alexa Fluor® 568 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab312761)
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab179434于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/210.
|
|
WB | (4) |
1/1000. Predicted molecular weight: 77 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified, use 1/100 - 1/250. |
说明 |
---|
Flow Cyt (Intra)
1/210. |
WB
1/1000. Predicted molecular weight: 77 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified, use 1/100 - 1/250. |
靶标
-
相关性
Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains. -
细胞定位
Cell Membrane, Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 7314 Human
- Entrez Gene: 22187 Mouse
- Entrez Gene: 192255 Rat
- SwissProt: P0CG47 Human
- SwissProt: P0CG48 Human
- SwissProt: P62979 Human
- SwissProt: P62987 Human
- SwissProt: P62988 Human
see all -
别名
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
see all
图片
-
All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (purified)
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 16-300 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Immunohistochemical staining of paraffin embedded human tonsil with purified ab179434 at a dilution of 1/500. The secondary antibody used is a HRP conjugated goat anti-rabbit (ab97051, 1/500). The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
-
All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/5000 dilution (purified)
Lane 1 : K6-linked-Ub2 recombinant protein
Lane 2 : K11-linked-Ub2 recombinant protein
Lane 3 : K29-linked-Ub2 recombinant protein
Lane 4 : K33-linked-Ub2 recombinant protein
Lane 5 : K48-linked-Ub2 recombinant protein
Lane 6 : K63-linked-Ub2 recombinant protein
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 16-70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Ubiquitin (linkage-specific K63) with purified ab179434 at 1/210 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
-
All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 16-300 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with unpurified ab179434 at 1/100 dilution.
-
All lanes : Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab179434) at 1/1000 dilution (unpurified)
Lane 1 : Ubiquitin recombinant protein
Lane 2 : K6-linked-Ub2 recombinant protein
Lane 3 : K11-linked-Ub2 recombinant protein
Lane 4 : K27-linked-Ub2 recombinant protein
Lane 5 : K29-linked-Ub2 recombinant protein
Lane 6 : K33-linked-Ub2 recombinant protein
Lane 7 : K48-linked-Ub2-7 recombinant protein
Lane 8 : K63-linked-Ub2-7 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 77 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (75)
ab179434 被引用在 75 文献中.
- Mevissen TET et al. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away. Cell Rep 42:112125 (2023). WB ; Xenopus laevis . PubMed: 36807144
- Deng Q et al. A detrimental role of NLRP6 in host iron metabolism during Salmonella infection. Redox Biol 49:102217 (2022). PubMed: 34942528
- Puangmalai N et al. Lysine 63-linked ubiquitination of tau oligomers contributes to the pathogenesis of Alzheimer's disease. J Biol Chem 298:101766 (2022). PubMed: 35202653
- Stormo AED et al. The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity. J Cell Biol 221:N/A (2022). PubMed: 35266954
- Li Y et al. An oxygen-adaptive interaction between SNHG12 and occludin maintains blood-brain barrier integrity. Cell Rep 39:110656 (2022). PubMed: 35417709