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Anti-Ubiquinol-Cytochrome C Reductase Core蛋白I抗体[16D10AD9AH5] (ab110252)

Reviews (1) Submit a question References (105)
Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

Key features and details

  • Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP

概述

  • 产品名称

    Anti-Ubiquinol-Cytochrome C Reductase Core蛋白I抗体[16D10AD9AH5]
    参阅全部 Ubiquinol-Cytochrome C Reductase Core Protein I 一抗

  • 描述

    小鼠单克隆抗体[16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core蛋白I

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • 阳性对照

    • Human, Cow, Rat or Mouse heart mitochondrial lysate, HepG2 mitochondrial lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HCT116

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110252于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
WB (1)
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 53 kDa.
说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
1/50.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 53 kDa.

靶标

图片

  • Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Western blot - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    All lanes : Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252) at 0.5 µg/ml

    Lane 1 : Human heart mitochondrial lysate
    Lane 2 : Cow heart mitochondrial lysate
    Lane 3 : Rat heart mitochondrial lysate
    Lane 4 : Mouse heart mitochondrial lysate
    Lane 5 : HepG2 mitochondrial lysate

    Predicted band size: 53 kDa



    Extra bands in the Mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
  • Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Immunocytochemistry/ Immunofluorescence - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)

    ab110252 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Flow Cytometry - Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252)
    Overlay histogram showing HepG2 cells stained with ab110252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献 (105)

发表研究结果有使用 ab110252？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110252 被引用在 105 文献中.

  • Viguier C  et al. Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet. Neuropharmacology 241:109730 (2023). WB ; Mouse . PubMed: 37758019
  • Van Haute L  et al. TEFM variants impair mitochondrial transcription causing childhood-onset neurological disease. Nat Commun 14:1009 (2023). PubMed: 36823193
  • Schuettpelz J  et al. The role of the mitochondrial outer membrane protein SLC25A46 in mitochondrial fission and fusion. Life Sci Alliance 6:N/A (2023). PubMed: 36977595
  • Potes Y  et al. Melatonin Alleviates the Impairment of Muscle Bioenergetics and Protein Quality Control Systems in Leptin-Deficiency-Induced Obesity. Antioxidants (Basel) 12:N/A (2023). PubMed: 38001815
  • Burgin H  et al. Loss of mitochondrial fatty acid β-oxidation protein short-chain Enoyl-CoA hydratase disrupts oxidative phosphorylation protein complex stability and function. FEBS J 290:225-246 (2023). PubMed: 35962613
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Western blot abreview for Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (Isolated Mouse Liver Mitochondria)
Loading amount
20 µg
Specification
Isolated Mouse Liver Mitochondria
Gel Running Conditions
Reduced Denaturing (10% Bis Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Dec 05 2011

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