Anti-Ubiquilin/UBQLN1抗体(ab3341)
Key features and details
- Rabbit polyclonal to Ubiquilin/UBQLN1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
概述
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产品名称
Anti-Ubiquilin/UBQLN1抗体 -
描述
兔多克隆抗体to Ubiquilin/UBQLN1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human, Chinese hamster -
免疫原
Synthetic peptide corresponding to Mouse Ubiquilin/UBQLN1 aa 2-18.
Sequence:AESAESGGPPGAQDSAA
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
| 说明 |
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IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
靶标
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功能
Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation. -
组织特异性
Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney. -
序列相似性
Contains 1 UBA domain.
Contains 1 ubiquitin-like domain. -
结构域
Binds PSEN1 and PSEN2 via its UBA domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 29979 Human
- Entrez Gene: 56085 Mouse
- Omim: 605046 Human
- SwissProt: Q9UMX0 Human
- SwissProt: Q8R317 Mouse
- Unigene: 9589 Human
- Unigene: 182053 Mouse
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别名
- DA41 antibody
- DSK2 antibody
- FLJ90054 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)Anti-Ubiquilin/UBQLN1 antibody (ab3341) + CHO (Chinese hamster ovary cell line) whole cell lysate
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab3341 被引用在 8 文献中.
- Feng X et al. Abnormally elevated ubiquilin‑1 expression in breast cancer regulates metastasis and stemness via AKT signaling. Oncol Rep 46:N/A (2021). PubMed: 34528694
- Liu Y et al. Attenuation of Ischemic Stroke-Caused Brain Injury by a Monoamine Oxidase Inhibitor Involves Improved Proteostasis and Reduced Neuroinflammation. Mol Neurobiol 57:937-948 (2020). PubMed: 31620993
- Zhang X et al. The impacts of ubiquilin 1 (UBQLN1) knockdown on cells viability, proliferation, and apoptosis are mediated by p53 in A549 lung cancer cells. J Thorac Dis 12:5887-5895 (2020). PubMed: 33209421
- Hu C et al. Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. J Clin Invest 128:5294-5306 (2018). PubMed: 30204128
- Adegoke OO et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . PubMed: 28598849
- González-Pérez P et al. Association of UBQLN1 mutation with Brown-Vialetto-Van Laere syndrome but not typical ALS. Neurobiol Dis 48:391-8 (2012). PubMed: 22766032
- Adusei DC et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . PubMed: 20470805
- Hiltunen M et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem 281:32240-53 (2006). ICC/IF ; Mouse . PubMed: 16945923
