重组Anti-UBF1抗体[EPR2742] (ab109011)
![Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)](https://www.abcam.cn/ps/products/109/ab109011/Images/ab109011-121575-anti-ubf1-antibody-epr2742-western-blot.jpg "Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2742] to UBF1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UBF1抗体[EPR2742]
参阅全部 UBF1 一抗 -
描述
兔单克隆抗体[EPR2742] to UBF1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, A431, MCF7 and JAR cell lysates; Human colon and breast tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR2742 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109011于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 89 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
靶标
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功能
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. -
序列相似性
Contains 6 HMG box DNA-binding domains. -
翻译后修饰
Phosphorylated and activated by PIK3CA. -
细胞定位
Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 7343 Human
- Entrez Gene: 21429 Mouse
- Entrez Gene: 25574 Rat
- Omim: 600673 Human
- SwissProt: P17480 Human
- SwissProt: P25976 Mouse
- SwissProt: P25977 Rat
- Unigene: 89781 Human
see all -
别名
- 90 kDa nucleolus organizer region autoantigen antibody
- Autoantigen NOR-90 antibody
- NOR 90 antibody
see all
图片
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Western blot - Anti-UBF1 antibody [EPR2742] (ab109011)All lanes : Anti-UBF1 antibody [EPR2742] (ab109011) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 89 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)](https://www.abcam.cn/ps/products/109/ab109011/Images/ab109011-121574-anti-ubf1-antibody-epr2742-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)ab109011 at 1/50 dilution staining UBF1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)](https://www.abcam.cn/ps/products/109/ab109011/Images/ab109011-121573-anti-ubf1-antibody-epr2742-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EPR2742] (ab109011)ab109011 at 1/50 dilution staining UBF1 in Human breast by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-UBF1 antibody [EPR2742] (ab109011)](https://www.abcam.cn/ps/products/109/ab109011/Images/ab109011-4-benefits-of-recombinant-antibodies.png)
Anti-UBF1 antibody [EPR2742] (ab109011)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109011 尚未被引用在任何文献中。