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重组RabMAb

重组Anti-UBF1抗体[EP2741Y] (ab75781)

Submit a review Submit a question References (3)
Western blot - Anti-UBF1 antibody [EP2741Y] (ab75781)
  • Flow Cytometry (Intracellular) - Anti-UBF1 antibody [EP2741Y] (ab75781)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EP2741Y] (ab75781)
  • Anti-UBF1 antibody [EP2741Y] (ab75781)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2741Y] to UBF1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-UBF1抗体[EP2741Y]
    参阅全部 UBF1 一抗

  • 描述

    兔单克隆抗体[EP2741Y] to UBF1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-Pmore details
    不适用于: IP

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa and A431 cell lysates, human breast carcinoma

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EP2741Y

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75781于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/30 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/30 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

  • 序列相似性

    Contains 6 HMG box DNA-binding domains.

  • 翻译后修饰

    Phosphorylated and activated by PIK3CA.

  • 细胞定位

    Nucleus > nucleolus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    图片

    • Western blot - Anti-UBF1 antibody [EP2741Y] (ab75781)
      Western blot - Anti-UBF1 antibody [EP2741Y] (ab75781)
      All lanes : Anti-UBF1 antibody [EP2741Y] (ab75781) at 1/500 dilution

      Lane 1 : (A) HeLa cell
      lysate
      Lane 2 : (B) A431 cell
      lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 89 kDa
      Observed band size: 89,89 kDa

    • Flow Cytometry (Intracellular) - Anti-UBF1 antibody [EP2741Y] (ab75781)
      Flow Cytometry (Intracellular) - Anti-UBF1 antibody [EP2741Y] (ab75781)

      Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EP2741Y] (ab75781)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBF1 antibody [EP2741Y] (ab75781)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-UBF1 antibody [EP2741Y] (ab75781)
      Anti-UBF1 antibody [EP2741Y] (ab75781)

    文献 (3)

    发表研究结果有使用 ab75781？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75781 被引用在 3 文献中.

    • Chen HF  et al. Inhibition of TAF1B impairs ribosome biosynthesis and suppresses cell proliferation in stomach adenocarcinoma through promoting c-MYC mRNA degradation. Heliyon 10:e23167 (2024). PubMed: 38169774
    • Stixová L  et al. UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res 27:41-55 (2019). PubMed: 30610403
    • Tchurikov NA  et al. Hot spots of DNA double-strand breaks in human rDNA units are produced in vivo. Sci Rep 6:25866 (2016). PubMed: 27160357

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