重组Anti-UBE4B抗体[S04-6C6] (ab317434)
![Western blot - Anti-UBE4B Antibody [S04-6C6] (ab317434)](https://www.abcam.cn/ps/products/317/ab317434/Images/ab317434-536655-anti-ube4b-antibody-s04-6c6-western-blot-hela-a549-hl60-u2os-u897mg-human.jpg "Western blot - Anti-UBE4B Antibody [S04-6C6] (ab317434)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S04-6C6] to UBE4B
- Suitable for: WB, ICC/IF
- Reacts with: Human
概述
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产品名称
Anti-UBE4B抗体[S04-6C6]
参阅全部 UBE4B 一抗 -
描述
兔单克隆抗体[S04-6C6] to UBE4B -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human UBE4B.
Database link: O95155 -
阳性对照
- WB: HeLa, A549, HL-60, U2OS and U897-MG cell lysate. ICC/IF: MCF-7 cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S04-6C6 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317434于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 146 kDa.
|
|
| ICC/IF |
1/20.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 146 kDa. |
|
ICC/IF
1/20. |
靶标
-
功能
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
组织特异性
Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen. -
序列相似性
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. -
翻译后修饰
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 10277 Human
- SwissProt: O95155 Human
- Unigene: 593974 Human
-
别名
- 4930551I19Rik antibody
- 4933406G05Rik antibody
- AU014668 antibody
see all
图片
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Western blot - Anti-UBE4B Antibody [S04-6C6] (ab317434)All lanes : Anti-UBE4B Antibody [S04-6C6] (ab317434) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : U2OS cell lysate
Lane 5 : U897-MG cell lysate
Predicted band size: 146 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-UBE4B Antibody [S04-6C6] (ab317434)](https://www.abcam.cn/ps/products/317/ab317434/Images/ab317434-536864-anti-ube4b-antibody-s04-6c6-immunocytochemistry-mcf7-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UBE4B Antibody [S04-6C6] (ab317434)Immunocytochemistry of UBE4B (green) in MCF-7 using ab317434 at dilution 1/20, and DAPI (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317434 尚未被引用在任何文献中。