为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    按产品类型
    蛋白质与肽
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Epigenetics and Nuclear Signaling Cell cycle Ubiquitin ligases

Anti-UBE4B抗体(ab97697)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (3)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-UBE4B antibody (ab97697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (ab97697)

Key features and details

  • Rabbit polyclonal to UBE4B
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

你可能会感兴趣

一抗
Product image
Anti-GRB10 antibody (ab154029)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-UBE4B抗体
    参阅全部 UBE4B 一抗
  • 描述

    兔多克隆抗体to UBE4B
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Cow, Zebrafish
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 740-1020 of Human UBE4B (NP_006039).

  • 阳性对照

    • HepG2 whole cell lysate (ab7900); CA922 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Ubiquitin ligases

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab97697于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
IHC-P (1)
1/100 - 1/500.
说明
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
IHC-P
1/100 - 1/500.

靶标

  • 功能

    Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
  • 组织特异性

    Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
  • 序列相似性

    Belongs to the ubiquitin conjugation factor E4 family.
    Contains 1 U-box domain.
  • 翻译后修饰

    Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession O95155 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 520833 Cow
    • Entrez Gene: 10277 Human
    • Entrez Gene: 63958 Mouse
    • Entrez Gene: 170784 Zebrafish
    • SwissProt: O95155 Human
    • SwissProt: Q9ES00 Mouse
    • Unigene: 593974 Human
    • Unigene: 393991 Mouse
    • Unigene: 415260 Mouse
    see all
  • 别名

    • 4930551I19Rik antibody
    • 4933406G05Rik antibody
    • AU014668 antibody
    • D4Bwg0973e antibody
    • E4 antibody
    • HDNB1 antibody
    • Homozygously deleted in neuroblastoma 1 antibody
    • KIAA0684 antibody
    • mKIAA0684 antibody
    • RP23 173D8.1 antibody
    • Ube4b antibody
    • UBE4B_HUMAN antibody
    • Ubiquitin conjugation factor E4 B antibody
    • Ubiquitin fusion degradation protein 2 antibody
    • UBOX3 antibody
    • UFD2 antibody
    • UFD2a antibody
    • Ufd2p antibody
    see all

图片

  • Western blot - Anti-UBE4B antibody (ab97697)
    Western blot - Anti-UBE4B antibody (ab97697)
    Anti-UBE4B antibody (ab97697) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 133 kDa



    5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (ab97697)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (ab97697)
    Immunohistochemical analysis of UBE4B in paraffin-embedded CA922 xenograft, using ab97697 at 1/500 dilution.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab97697?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97697 被引用在 3 文献中.

  • Zhu L  et al. A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. EMBO Mol Med 14:e14552 (2022). PubMed: 35174975
  • Gireud-Goss M  et al. The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion. Mol Cell Neurosci 108:103542 (2020). PubMed: 32841720
  • Woodfield SE  et al. Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B. Genes Cancer 7:13-26 (2016). WB, IHC . PubMed: 27014418

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-UBE4B antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina, Cryosections)
Antigen retrieval step
None
Permeabilization
Yes - 0,5% Triton-x-100
Specification
Retina, Cryosections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Lisa Janina Klotz

Verified customer

提交于 Mar 28 2018

Question

In continuation to our previous correspondences, this time the customer wishes to proceed with the test of  ab97697 in ICC/IF with U2OS that were silenced with UBE4B siRNA. Please issue the discount code.                 Thanks in advance for your reply.                 Have a nice week.

Read More

Abcam community

Verified customer

Asked on Nov 28 2011

Answer

Thank you for your message and reply. I am very pleased to hear the customer would like to accept our offer and test ab97697 in ICC-IF. SiRNA data will be particularly useful to us and other customers. DISCOUNT CODE: ##### Expiration date: ###### This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

Read More

Abcam Scientific Support

回复于 Nov 28 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有