重组Anti-Ube2N / Ubc13抗体[S05-5B0] (ab317433)
![Western blot - Anti-Ube2N / Ubc13 Antibody [S05-5B0] (ab317433)](https://www.abcam.cn/ps/products/317/ab317433/Images/ab317433-1-anti-ube2n-ubc13-antibody-s05-5b0-western-blot-hl60-c6-human-rat.jpg "Western blot - Anti-Ube2N / Ubc13 Antibody [S05-5B0] (ab317433)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [[S05-5B0] to Ube2N / Ubc13
- Suitable for: WB
- Reacts with: Rat, Human
概述
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产品名称
Anti-Ube2N / Ubc13抗体[S05-5B0]
参阅全部 Ube2N / Ubc13 一抗 -
描述
兔单克隆抗体[[S05-5B0] to Ube2N / Ubc13 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Ube2N/ Ubc13.
Database link: P61088 -
阳性对照
- WB: HL-60 and C6 cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
[S05-5B0 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317433于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 17 kDa.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 17 kDa. |
靶标
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功能
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻译后修饰
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7334 Human
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
- Unigene: 165094 Rat
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别名
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
图片
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Western blot - Anti-Ube2N / Ubc13 Antibody [S05-5B0] (ab317433)All lanes : Anti-Ube2N / Ubc13 Antibody [S05-5B0] (ab317433) at 1/1000 dilution
Lane 1 : HL-60 cell lysate
Lane 2 : C6 cell lysate
Predicted band size: 17 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317433 尚未被引用在任何文献中。