重组Anti-Ube2N / Ubc13抗体[EPR5162] (ab109286)
![Western blot - Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)](https://www.abcam.cn/ps/products/109/ab109286/Images/ab109286-122022-anti-ube2n-ubc13-antibody-epr5162-western-blot.jpg "Western blot - Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5162] to Ube2N / Ubc13
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ube2N / Ubc13抗体[EPR5162]
参阅全部 Ube2N / Ubc13 一抗 -
描述
兔单克隆抗体[EPR5162] to Ube2N / Ubc13 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human testis tissue. Jurkat, Daudi and Human heart lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5162 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt or IP.
靶标
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功能
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻译后修饰
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
see all -
别名
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
图片
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Western blot - Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)All lanes : Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)](https://www.abcam.cn/ps/products/109/ab109286/Images/ab109286-122021-anti-ube2n-ubc13-antibody-epr5162-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109286 at 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)](https://www.abcam.cn/ps/products/109/ab109286/Images/ab109286-3-benefits-of-recombinant-antibodies.png)
Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab109286 被引用在 5 文献中.
- Dickens JA et al. Novel insights into surfactant protein C trafficking revealed through the study of a pathogenic mutant. Eur Respir J 59:N/A (2022). PubMed: 34049951
- Morale MG et al. HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis. Sci Rep 8:3476 (2018). WB . PubMed: 29472602
- Hu L et al. Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity. Nat Commun 8:814 (2017). PubMed: 28993672
- Yang Z et al. A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18. Mol Cell Proteomics 16:469-484 (2017). PubMed: 28087594
- Wang C et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). PubMed: 27082114