AB109507

重组Anti-UBE2M/UBC12抗体[EPR5333]

Anti-UBE2M/UBC12 antibody [EPR5333]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(17 Publications)

Rabbit Recombinant Monoclonal UBE2M/UBC12 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 17 publications.

查看别名

UBC12, UBE2M, NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, Ubiquitin-conjugating enzyme E2 M

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling UBE2M/UBC12 with purified ab109507 at 1/250 dilution (8 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

Immunohistochemical analysis of paraffin-embedded human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-UBE2M/UBC12 antibody [EPR5333] (AB109507)

All lanes:

Western blot - Anti-UBE2M/UBC12 antibody [EPR5333] (ab109507) at 1/10000 dilution

Lane 1:

HEK-293T cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Predicted band size: 21 kDa

Observed band size: 20 kDa

false

不同偶联物与剂型 (2)

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-UBE2M/UBC12 antibody [EPR5333]
Carrier free

Anti-UBE2M/UBC12 antibody [EPR5333] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5333

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Flow Cyt (Intra), ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

UBE2M also called UBC12 is an E2 enzyme involved in the process of neddylation which is a type of ubiquitin-like modification. The protein has a molecular mass of approximately 22 kDa. It activates and transfers NEDD8 a ubiquitin-like protein to specific substrates. UBE2M is expressed in various tissues with high expression levels in the liver muscle and kidney tissues. Its precise mechanical role is connecting to NEDD8 and collaborating with E3 ligases to modify substrates.

Biological function summary

E2 conjugating enzyme such as UBE2M functions in the regulation of the cell cycle signal transduction and other cellular processes. It is important in the neddylation pathway where it facilitates the covalent attachment of the NEDD8 molecule to Cullin proteins. UBE2M associates with the cullin-RING ligase complex assisting in the alteration of protein stability and function within cells. The proper operation of this enzyme is essential for maintaining cellular homeostasis.

Pathways

Neddylation modulates ubiquitination and acts as a regulator in protein degradation pathways. UBE2M occupies a central role in the neddylation pathway which intersects with the ubiquitin-proteasome system involving proteins like Cullin and NEDD8. Both neddylation and ubiquitination pathways share overlapping components and functions making UBE2M critical in selective protein degradation impacting processes such as DNA replication and repair and response to stress.

The incorrect function of UBE2M has links to cancer and neurodegenerative diseases. In cancer misregulation of the neddylation pathway can lead to unregulated cell growth and survival due to the aberrant degradation of key proteins. Abnormal UBE2M activity impacts the regulation of the tumor suppressor protein p53 through interaction with other neddylation-related proteins affecting cell cycle control. In neurodegenerative diseases alterations in protein homeostasis and aggregation occur related to the dysregulation of neddylation influencing proteins involved in neuronal function.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

See full target information UBE2M

style

left-column

文献 (17)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108085 PubMed39675717

2024

Inhibition of the neddylation E2 enzyme UBE2M in macrophages protects against E. coli-induced sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuehuan Wen,Songjie Bai,Guirun Xiong,Huiqing Xiu,Jiahui Li,Jie Yang,Qing Yu,Bingyu Li,Ruomeng Hu,Lanxin Cao,Zhijian Cai,Shufang Zhang,Gensheng Zhang

Nature communications 15:10536 PubMed39627198

2024

CRL3 E3 ligase facilitates ubiquitin-mediated degradation of oncogenic SRX to suppress colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhu,Liangshan Li,Yuanyuan Chen,Yongfu Pan,Wenjuan Zhang,Lihui Li,Lili Cai,Xiaoxue Zhao,Hu Zhao,Shiwen Wang,Lijun Jia

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305907 PubMed38126621

2023

NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer by Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yilei Ma,Xucheng Huang,Yanzhong Wang,Yinjiao Lei,Jinwei Yu,Shaobo Yu,Yuzhen Gao,Jun Yang,Feng Zhao,Haitao Yu,Jin Zeng,Yadong Chu,Min Yang,Guoli Li,Xinyou Xie,Jun Zhang

Nature communications 14:6774 PubMed37891180

2023

N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvia Varland,Rui Duarte Silva,Ine Kjosås,Alexandra Faustino,Annelies Bogaert,Maximilian Billmann,Hadi Boukhatmi,Barbara Kellen,Michael Costanzo,Adrian Drazic,Camilla Osberg,Katherine Chan,Xiang Zhang,Amy Hin Yan Tong,Simonetta Andreazza,Juliette J Lee,Lyudmila Nedyalkova,Matej Ušaj,Alexander J Whitworth,Brenda J Andrews,Jason Moffat,Chad L Myers,Kris Gevaert,Charles Boone,Rui Gonçalo Martinho,Thomas Arnesen

Cell chemical biology 30:394-402.e9 PubMed36898369

2023

Chemoproteomics-enabled discovery of a covalent molecular glue degrader targeting NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A King,Yoojin Cho,Nathan S Hsu,Dustin Dovala,Jeffrey M McKenna,John A Tallarico,Markus Schirle,Daniel K Nomura

Bioscience reports 42: PubMed35880551

2022

Inhibition of NEDD8 NEDDylation induced apoptosis in acute myeloid leukemia cells via p53 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Chen,Ling Sun

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21800 PubMed34324733

2021

Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dacai Xu,Jiawen Wu,Jinghong Chen,Liling Jiang,Juan Chen,Wenhao Bao,Xin Chen,Qianqian Yang,Xiaolan Zhang,Leyi Yao,Huabo Su,Jinbao Liu

Cell death & disease 11:975 PubMed33184273

2020

Induction of NEDD8-conjugating enzyme E2 UBE2F by platinum protects lung cancer cells from apoptosis and confers to platinum-insensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Zhou,Jin Zhu,Wangyang Chen,Yanyu Jiang,Tao Hu,Yinxia Wang,Xiaoling Ye,Mengxi Zhan,Chenghao Ji,Zhuoming Xu,Xinran Wang,Yuanlong Gu,Lijun Jia

Biochemical and biophysical research communication 529:970-976 PubMed32819607

2020

Ubiquitin conjugating enzyme E2 M promotes apoptosis in osteoarthritis chondrocytes via Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Ba,Xiaohui Ni,Junlong Yu,Guoyou Zou,Hao Zhu

Cell death & disease 11:400 PubMed32457294

2020

HUWE1-dependent DNA-PKcs neddylation modulates its autophosphorylation in DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Zongpei Guo,Shaozheng Wang,Ying Xie,Yang Han,Sai Hu,Hua Guan,Dafei Xie,Chenjun Bai,Xiaodan Liu,Yongqing Gu,Ping-Kun Zhou,Teng Ma

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-UBE2M/UBC12抗体[EPR5333]