重组Anti-Ube2L6抗体[EPR4267(2)] (ab109086)
![Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)](https://www.abcam.cn/ps/products/109/ab109086/Images/ab109086-121648-anti-ube2l6-antibody-epr42672-western-blot.jpg "Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4267(2)] to Ube2L6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Ube2L6抗体[EPR4267(2)]
参阅全部 Ube2L6 一抗 -
描述
兔单克隆抗体[EPR4267(2)] to Ube2L6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal thymus, Human lymph node, Daudi, and MOLT4 lysates; Human kidney and uterus tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4267(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109086于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
靶标
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功能
Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. -
组织特异性
Present in natural killer cells (at protein level). -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻译后修饰
ISGylated. - Information by UniProt
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数据库链接
- Entrez Gene: 9246 Human
- Omim: 603890 Human
- SwissProt: O14933 Human
- Unigene: 425777 Human
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别名
- MGC40331 antibody
- Retinoic acid induced gene B protein antibody
- Retinoic acid-induced gene B protein antibody
see all
图片
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Western blot - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)All lanes : Anti-Ube2L6 antibody [EPR4267(2)] (ab109086) at 1/1000 dilution
Lane 1 : Human fetal thymus tissue lysate
Lane 2 : Human lymph node tissue lysate
Lane 3 : Daudi cell lysate
Lane 4 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)](https://www.abcam.cn/ps/products/109/ab109086/Images/ab109086-121647-anti-ube2l6-antibody-epr42672-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)ab109086 at 1/250 dilution staining Ube2L6 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)](https://www.abcam.cn/ps/products/109/ab109086/Images/ab109086-121646-anti-ube2l6-antibody-epr42672-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)ab109086 at 1/250 dilution staining Ube2L6 in Human uterus by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)](https://www.abcam.cn/ps/products/109/ab109086/Images/ab109086-4-benefits-of-recombinant-antibodies.png)
Anti-Ube2L6 antibody [EPR4267(2)] (ab109086)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109086 被引用在 4 文献中.
- Oki N et al. Curcumin partly prevents ISG15 activation via ubiquitin-activating enzyme E1-like protein and decreases ISGylation. Biochem Biophys Res Commun 625:94-101 (2022). PubMed: 35952613
- Ke M Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer. Oxid Med Cell Longev 2022:3124122 (2022). PubMed: 36567857
- Zhang Q et al. UHRF1 epigenetically down-regulates UbcH8 to inhibit apoptosis in cervical cancer cells. Cell Cycle 17:300-308 (2018). PubMed: 29157076
- Sooryanarain H et al. ISG15 Modulates Type I Interferon Signaling and the Antiviral Response during Hepatitis E Virus Replication. J Virol 91:N/A (2017). PubMed: 28724761