Anti-UBE2E3抗体[OTI4B4] (ab128098)
Key features and details
- Mouse monoclonal [OTI4B4] to UBE2E3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-UBE2E3抗体[OTI4B4]
参阅全部 UBE2E3 一抗 -
描述
小鼠单克隆抗体[OTI4B4] to UBE2E3 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human UBE2E3 aa 1-207. (NP_006348) produced in E.coli.
Sequence:MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTK LSSKTTAKLSTSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGP PGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILK DNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQ WTKRYAT
Database link: Q969T4 -
阳性对照
- WB: HepG2 extracts; HEK-293T cells transiently transfected with the UBE2E3 cDNA. IHC-P: Human endometrium adenocarcinoma tissue. ICC/IF: HepG2 cells. Flow Cyt (Intra): HeLa and Jurkat cells; HEK-293T cells transfected with UBE2E3 overexpressing plasmid.
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常规说明
Clone OTI4B4 (formerly 4B4).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI4B4 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128098于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 22 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/2000. Predicted molecular weight: 22 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. -
组织特异性
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
细胞定位
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 10477 Human
- Omim: 604151 Human
- SwissProt: Q969T4 Human
- Unigene: 470804 Human
- Unigene: 567831 Human
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别名
- Homologous to yeast UBC4/5 antibody
- UB2E3_HUMAN antibody
- UBC E4 antibody
see all
图片
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Anti-UBE2E3 antibody [OTI4B4] (ab128098) at 1/200 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) extracts at 10 µg
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE2E3 antibody [OTI4B4] (ab128098)
Paraffin-embedded human endometrium adenocarcinoma tissue stained for UBE2E3 with ab128098 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for UBE2E3 using ab128098 at a 1/100 dilution in ICC/IF.
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Flow cytometry (Intracellular) analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, using anti-UBE2E3 antibody (ab128098) in Red at 1/100 dilution, compared to a nonspecific negative control antibody in Blue.
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Flow cytometry analysis (Intracellular) of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, using anti-UBE2E3 antibody (ab128098) in Red at 1/100 dilution, compared to a nonspecific negative control antibody in Blue.
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Flow cytometry analysis (Intracellular) of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either UBE2E3 overexpressing plasmid (Red) or empty vector control plasmid (Blue) and immunostained by anti-UBE2E3 antibody (ab128098) at 1/100 dilution.
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All lanes : Anti-UBE2E3 antibody [OTI4B4] (ab128098) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY UBE2E3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab128098 被引用在 1 文献中.
- King LE et al. Genes regulating membrane-associated E-cadherin and proliferation in adenomatous polyposis coli mutant colon cancer cells: High content siRNA screen. PLoS One 15:e0240746 (2020). PubMed: 33057364