重组Anti-UBE2E2抗体[EPR13003(B)] (ab177485)
![Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)](https://www.abcam.cn/ps/products/177/ab177485/Images/ab177485-205412-anti-ube2e2-antibody-epr13003b-western-blot.jpg "Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13003(B)] to UBE2E2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UBE2E2抗体[EPR13003(B)]
参阅全部 UBE2E2 一抗 -
描述
兔单克隆抗体[EPR13003(B)] to UBE2E2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human UBE2E2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96LR5 -
阳性对照
- HeLa, LNCaP, 293T and Jurkat whole cell lysate (ab7899).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13003(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177485于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
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| 说明 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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数据库链接
- Entrez Gene: 7325 Human
- Entrez Gene: 218793 Mouse
- Entrez Gene: 361013 Rat
- Omim: 602163 Human
- SwissProt: Q96LR5 Human
- SwissProt: Q91W82 Mouse
- Unigene: 475688 Human
- Unigene: 595802 Human
see all -
别名
- FLJ25157 antibody
- UB2E2_HUMAN antibody
- UBC4/5 homolog yeast antibody
see all
图片
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Western blot - Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)All lanes : Anti-UBE2E2 antibody [EPR13003(B)] (ab177485) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa -
![Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)](https://www.abcam.cn/ps/products/177/ab177485/Images/ab177485-2-benefits-of-recombinant-antibodies.png)
Anti-UBE2E2 antibody [EPR13003(B)] (ab177485)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab177485 被引用在 2 文献中.
- Xue X et al. YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription. Cell Death Discov 8:59 (2022). PubMed: 35149670
- Gan N et al. Legionella pneumophila inhibits immune signalling via MavC-mediated transglutaminase-induced ubiquitination of UBE2N. Nat Microbiol 4:134-143 (2019). PubMed: 30420781