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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation

Anti-UBE2C抗体(ab3935)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-UBE2C antibody (ab3935)
  • Immunoprecipitation - Anti-UBE2C antibody (ab3935)

Key features and details

  • Goat polyclonal to UBE2C
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-UBE2C抗体
    参阅全部 UBE2C 一抗
  • 描述

    山羊多克隆抗体to UBE2C
  • 宿主

    Goat
  • 经测试应用

    适用于: WB, IPmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide: QETYSKQVTSQEP, corresponding to C terminal amino acids 167-179 of Human UBE2C.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E2 Ubiquitin Conjugating Enzymes
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E2 Enzymes

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3935于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).

A 1 hour primary incubation is recommended for this product.

IP
Use at an assay dependent concentration. (See Abreview)
说明
WB
Use a concentration of 2.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21.5 kDa).

A 1 hour primary incubation is recommended for this product.

IP
Use at an assay dependent concentration. (See Abreview)

靶标

  • 功能

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
  • 通路

    Protein modification; protein ubiquitination.
  • 序列相似性

    Belongs to the ubiquitin-conjugating enzyme family.
  • 翻译后修饰

    Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.
  • Target information above from: UniProt accession O00762 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 11065 Human
    • Omim: 605574 Human
    • SwissProt: O00762 Human
    • Unigene: 93002 Human
    • 别名

      • Cyclin selective ubiquitin carrier protein antibody
      • dJ447F3.2 antibody
      • Mitotic specific ubiquitin conjugating enzyme antibody
      • UB E2C antibody
      • UBCH 10 antibody
      • UbcH10 antibody
      • UBE 2C antibody
      • Ube2c antibody
      • UBE2C_HUMAN antibody
      • Ubiquitin carrier protein C antibody
      • Ubiquitin carrier protein E2 C antibody
      • Ubiquitin carrier protein E2C antibody
      • Ubiquitin conjugating enzyme E2 C antibody
      • Ubiquitin conjugating enzyme E2C antibody
      • Ubiquitin protein ligase C antibody
      • Ubiquitin-conjugating enzyme E2 C antibody
      • Ubiquitin-protein ligase C antibody
      see all

    图片

    • Western blot - Anti-UBE2C antibody (ab3935)
      Western blot - Anti-UBE2C antibody (ab3935)

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

      ab3935 at a 2.5µg/ml concentration staining approximately 20 kDa UBE2C by Western blot (ECL):

      Lane 1 = pCDNA3 transfected U2OS whole cell lysate (2µl).
      Lane 2 = pCDNA3 MycUbcH10 transfected whole cell lysate (2µl).
      Lane 3 = Nocodazole arrested HeLa cell whole cell lysate (20µl).
      Lane 4 = Asynchronous HeLa cell whole cell lysate (20µl).

      For a more detailed descri
    • Immunoprecipitation - Anti-UBE2C antibody (ab3935)
      Immunoprecipitation - Anti-UBE2C antibody (ab3935)

      ab3935 staining approximately 20 kDa human UBE2 after immmunoprecipitation from HeLa whole cell lysate (106 cells/ml) using 40µl of ab3935 saturated protein G dynabeads, by Western blot (ECL):

      Lane 1 = IP from Noc arrested HeLa cell extract (beads only).
      Lane 2 = IP from Noc arrested HeLa cell extract (goat whole serum coupled to beads).
      Lane 3 = IP from Noc arrested HeLa cell extract (ab3935 coupled to beads)
      Lane 4 = Noc arrested HeLa whole cell lysate.

      For a more detailed description, please click the reviews tab at the top of the page and see the review dated 25/02/03.

    实验方案

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (3)

    发表研究结果有使用 ab3935?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3935 被引用在 3 文献中.

    • Zhang Y  et al. Inhibition of microRNA-17/20a suppresses cell proliferation in gastric cancer by modulating UBE2C expression. Oncol Rep 33:2529-36 (2015). PubMed: 25760688
    • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). PubMed: 23911795
    • Ieta K  et al. Identification of overexpressed genes in hepatocellular carcinoma, with special reference to ubiquitin-conjugating enzyme E2C gene expression. Int J Cancer 121:33-8 (2007). PubMed: 17354233

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