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AB108929

重组Anti-Ube1L抗体[EPR4270]

Anti-Ube1L antibody [EPR4270]

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(2 Publications)

Rabbit Recombinant Monoclonal Ube1L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

查看别名

UBE1L, UBE2, UBA7, Ubiquitin-like modifier-activating enzyme 7, Ubiquitin-activating enzyme 7, D8, Ubiquitin-activating enzyme E1 homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (AB108929)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (AB108929)

Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)
  • WB

Unknown

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)

All lanes:

Western blot - Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

Fetal liver lysate at 10 µg

Predicted band size: 111 kDa

false

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)
  • WB

Lab

Western blot - Anti-Ube1L antibody [EPR4270] (AB108929)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution

Lane 1:

GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)

Lane 2:

GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 111 kDa

Observed band size: 146 kDa,166 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Ube1L antibody [EPR4270] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4270

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Ube1L also known as ubiquitin-activating enzyme E1-like plays an important role in the ubiquitin-proteasome system by activating ubiquitin molecules. With a molecular mass of approximately 117 kDa Ube1L functions as the initial enzyme in the ubiquitination cascade catalyzing the formation of a thioester bond with ubiquitin. This process is important for the tagging of proteins for degradation. Ube1L exhibits widespread expression in various tissues indicating its importance in diverse cellular activities.
Biological function summary

This protein participates actively in the regulation of protein homeostasis. Ube1L helps to manage protein turnover and maintains protein quality control by marking damaged or unneeded proteins for degradation via the proteasome. Although Ube1L functions primarily as a standalone enzyme it cooperates closely with ubiquitin-conjugating and -ligating enzymes (E2s and E3s) to achieve precise ubiquitination of target proteins. This cooperation is critical for cellular processes like cell cycle regulation and response to stress.

Pathways

Ube1L is a significant element in the ubiquitin-proteasome pathway which is essential for protein degradation and turnover. It also contributes to the regulation of interferon signaling by modifying proteins such as ISG15 a ubiquitin-like protein involved in antiviral response. Related proteins such as UBE2L6 and UBE2E1 serve as E2 conjugating enzymes that interact with Ube1L further influencing these pathways by facilitating the transfer of activated ubiquitin.

Ube1L has been implicated in certain types of cancers and viral infections. Alterations in the expression or function of Ube1L can lead to disrupted protein degradation contributing to cancer progression through the accumulation of oncogenic proteins. Ube1L is also connected to proteins like ISG15 and USP18 which are involved in host defense mechanisms against viral infections. Dysregulation in these contexts can result in inadequate antiviral responses or improper regulation of cell proliferation.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation (PubMed : 27545325). Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus (PubMed : 16254333, PubMed : 19073728, PubMed : 29056542, PubMed : 29743376, PubMed : 37722521). For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition (PubMed : 20133869). ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling (PubMed : 27564865).
See full target information UBA7
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8476 PubMed41006228

2025

EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Maric,Sandra Segura-Bayona,Raviprasad Kuthethur,Tohru Takaki,Valerie Borel,Tyler H Stanage,Miroslav P Ivanov,Nishita Parnandi,Graeme Hewitt,Rhona Millar,Carmen S Fonseca,Harshil Patel,Miriam Llorian,Scott Warchal,Michael Howell,Arnab Ray Chaudhuri,Panagiotis Kotsantis,Simon J Boulton

Journal of virology 98:e0085524 PubMed39120134

2024

SARS-CoV-2 papain-like protease inhibits ISGylation of the viral nucleocapsid protein to evade host anti-viral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Aulia Fitri Rhamadianti,Takayuki Abe,Tomohisa Tanaka,Chikako Ono,Hisashi Katayama,Yoshiteru Makino,Lin Deng,Chieko Matsui,Kohji Moriishi,Fumi Shima,Yoshiharu Matsuura,Ikuo Shoji
View all publications
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替代产品

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube1L + E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR4269(2)] (AB133479)

  • 4
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