重组Anti-UBA52抗体[EPR4547] (ab109230)
![Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (ab109230)](https://www.abcam.cn/ps/products/109/ab109230/Images/ab109230-236057-anti-uba52-antibody-epr4547-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (ab109230)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4547] to UBA52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-UBA52抗体[EPR4547]
参阅全部 UBA52 一抗 -
描述
兔单克隆抗体[EPR4547] to UBA52 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human placenta, Raji, fetal kidney, 293T and Jurkat lysates IHC-P: Human brain tissue IF/ICC: HepG2 cell line.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4547 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109230于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 15 kDa).
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
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| ICC/IF |
1/250 - 1/500.
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| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 15 kDa). |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
|
ICC/IF
1/250 - 1/500. |
靶标
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相关性
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. -
细胞定位
Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm -
数据库链接
- Entrez Gene: 7311 Human
- Entrez Gene: 22186 Mouse
- Entrez Gene: 64156 Rat
- Omim: 191321 Human
- SwissProt: P62987 Human
- SwissProt: P62984 Mouse
- SwissProt: P62986 Rat
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别名
- 60S ribosomal protein L40 antibody
- CEP52 antibody
- HUBCEP52 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (ab109230)
ab109230 stained HepG2 cells. The cells were 4% formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-UBA52 antibody [EPR4547] (ab109230)](https://www.abcam.cn/ps/products/109/ab109230/Images/ab109230-121899-anti-uba52-antibody-epr4547-western-blot.jpg)
Western blot - Anti-UBA52 antibody [EPR4547] (ab109230)All lanes : Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Raji cell lysate
Lane 3 : Fetal kidney lysate
Lane 4 : 293T cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 15 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (ab109230)](https://www.abcam.cn/ps/products/109/ab109230/Images/ab109230-121898-anti-uba52-antibody-epr4547-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (ab109230)Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue, using ab109230 at a 1/500 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-UBA52 antibody [EPR4547] (ab109230)](https://www.abcam.cn/ps/products/109/ab109230/Images/ab109230-4-benefits-of-recombinant-antibodies.png)
Anti-UBA52 antibody [EPR4547] (ab109230)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109230 被引用在 1 文献中.
- Kobayashi M et al. The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development. Sci Rep 6:36780 (2016). PubMed: 27829658