AB109230

重组Anti-UBA52抗体[EPR4547]

Anti-UBA52 antibody [EPR4547]

20ul selling size
RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal UBA52 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

查看别名

UBCEP2, UBA52, Ubiquitin-ribosomal protein eL40 fusion protein, CEP52, Ubiquitin A-52 residue ribosomal protein fusion product 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (AB109230)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4547] (AB109230)

Immunohistochemical analysis of UBA52 in paraffin embedded Human brain tissue using ab109230 at a 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (AB109230)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4547] (AB109230)

ab109230 stained HepG2 cells. The cells were 4% formaldehdye fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109230 at 1/200) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96899 Dylight 488 goat anti-rabbit IgG used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Unknown

Western blot - Anti-UBA52 antibody [EPR4547] (AB109230)

All lanes:

Western blot - Anti-UBA52 antibody [EPR4547] (ab109230) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Fetal kidney lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 15 kDa

Observed band size: 10 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-UBA52 antibody [EPR4547] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4547

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

UBA52 also known as Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 is a protein fusion of ubiquitin and ribosomal protein L40. It has a mass of approximately 15 kDa. UBA52 expresses widely in various tissues including the liver brain and heart playing a role in protein synthesis and ubiquitination. The ubiquitin domain of UBA52 gets translated and cleaved to participate in the ubiquitin-proteasome system while the ribosomal component integrates into the 60S subunit of the ribosome.

Biological function summary

UBA52 functions as both a ribosomal protein and a source of ubiquitin. This dual role enables it to participate in protein synthesis as part of the ribosome. Additionally it provides ubiquitin required for post-translational modification of proteins affecting protein stability and degradation. UBA52 is part of the ribosome complex and contributes to efficient protein translation while its ubiquitin segment influences protein turnover and cellular response to stress.

Pathways

UBA52 plays roles in protein translation and ubiquitin-mediated proteolysis. These pathways are essential for maintaining cellular homeostasis. In the context of protein synthesis UBA52 associates with other ribosomal proteins and supports the assembly of the ribosome facilitating mRNA translation. In the ubiquitin-proteasome pathway UBA52 contributes ubiquitin moieties used to tag proteins for degradation working with E2 and E3 ligases such as UBE2K and HUWE1 important for regulated proteolysis.

UBA52 is linked to conditions involving protein synthesis defects and improper protein degradation including cancer and neurodegenerative disorders. Changes in UBA52 expression or mutations can disrupt ribosome function affecting cellular proliferation and survival in cancers. Additionally impaired ubiquitin signaling has implications in neurodegenerative diseases where protein aggregates form due to faulty degradation. UBA52's connection to pathways with proteins like p53 in cancer and Parkin in neurodegeneration further illustrates its relevance across different pathologies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ubiquitin. Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked : Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in proteotoxic stress response and cell cycle; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.. Large ribosomal subunit protein eL40. Component of the 60S subunit of the ribosome (PubMed : 23169626, PubMed : 23636399, PubMed : 32669547, PubMed : 39048817, PubMed : 39103523). Ribosomal protein L40 is essential for translation of a subset of cellular transcripts, and especially for cap-dependent translation of vesicular stomatitis virus mRNAs (PubMed : 23169626, PubMed : 23636399, PubMed : 32669547, PubMed : 39048817, PubMed : 39103523).

See full target information UBA52

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 632:656-663 PubMed39048817

2024

SMYD5 methylation of rpL40 links ribosomal output to gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Juhyung Park,Jibo Wu,Krzysztof J Szkop,Jinho Jeong,Predrag Jovanovic,Dylan Husmann,Natasha M Flores,Joel W Francis,Ying-Jiun C Chen,Ana Morales Benitez,Emily Zahn,Shumei Song,Jaffer A Ajani,Linghua Wang,Kamini Singh,Ola Larsson,Benjamin A Garcia,Ivan Topisirovic,Or Gozani,Pawel K Mazur

Molecular cell 84:1753-1763.e7 PubMed38508183

2024

FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Joel William Francis,Simone Hausmann,Sabeen Ikram,Kunlun Yin,Robert Mealey-Farr,Natasha Mahealani Flores,Annie Truc Trinh,Tourkian Chasan,Julia Thompson,Pawel Karol Mazur,Or Gozani

Scientific reports 6:36780 PubMed27829658

2016

The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Kobayashi,Shigeru Oshima,Chiaki Maeyashiki,Yoichi Nibe,Kana Otsubo,Yu Matsuzawa,Yasuhiro Nemoto,Takashi Nagaishi,Ryuichi Okamoto,Kiichiro Tsuchiya,Tetsuya Nakamura,Mamoru Watanabe

View all publications

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重组Anti-UBA52抗体[EPR4547]