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重组RabMAb

重组Anti-UBA52抗体[EPR4546] (ab109227)

Submit a review Q&A (1)References (9)
Western blot - Anti-UBA52 antibody [EPR4546] (ab109227)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4546] (ab109227)
  • Flow Cytometry (Intracellular) - Anti-UBA52 antibody [EPR4546] (ab109227)
  • Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4546] (ab109227)
  • Anti-UBA52 antibody [EPR4546] (ab109227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4546] to UBA52
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

概述

  • 产品名称

    Anti-UBA52抗体[EPR4546]
    参阅全部 UBA52 一抗

  • 描述

    兔单克隆抗体[EPR4546] to UBA52

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Fetal kidney, Human placenta, 293T and JAR cell lysates. Human brain tissue. SH-SY-5Y cells. Flow Cyt (intra): HepG2 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109227于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/2500.
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
说明
Flow Cyt (Intra)
1/2500.
WB
1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.

靶标

  • 相关性

    Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.

  • 细胞定位

    Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm

  • 数据库链接

    • 别名

      • 60S ribosomal protein L40 antibody
      • CEP52 antibody
      • HUBCEP52 antibody
      • L40 antibody
      • MGC126879 antibody
      • MGC57125 antibody
      • RPL40 antibody
      • UBA 52 antibody
      • Ubiquitin 52 amino acid fusion protein antibody
      • Ubiquitin 60S ribosomal protein L40 antibody
      • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
      • Ubiquitin carboxyl extension protein 52 antibody
      • Ubiquitin CEP52 antibody
      see all

    图片

    • Western blot - Anti-UBA52 antibody [EPR4546] (ab109227)
      Western blot - Anti-UBA52 antibody [EPR4546] (ab109227)
      All lanes : Anti-UBA52 antibody [EPR4546] (ab109227) at 1/1000 dilution

      Lane 1 : Fetal kidney tissue lysate
      Lane 2 : Human placenta tissue lysate
      Lane 3 : 293T cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 15 kDa
      Observed band size: 10 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4546] (ab109227)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBA52 antibody [EPR4546] (ab109227)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/500 ab109227.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-UBA52 antibody [EPR4546] (ab109227)
      Flow Cytometry (Intracellular) - Anti-UBA52 antibody [EPR4546] (ab109227)

      Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling UBA52 (red) with purified ab109227 at a 1/2500 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4546] (ab109227)
      Immunocytochemistry/ Immunofluorescence - Anti-UBA52 antibody [EPR4546] (ab109227)
      Immunofluorescent staining of SH-SY-5Y cells using ab109227 at 1/250
    • Anti-UBA52 antibody [EPR4546] (ab109227)
      Anti-UBA52 antibody [EPR4546] (ab109227)

    文献 (9)

    发表研究结果有使用 ab109227？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109227 被引用在 9 文献中.

    • Xue M  et al. Loss of Paip1 causes translation reduction and induces apoptotic cell death through ISR activation and Xrp1. Cell Death Discov 9:288 (2023). PubMed: 37543696
    • Hopes T  et al. Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching. Nucleic Acids Res 50:2240-2257 (2022). PubMed: 34283226
    • Ma C  et al. Ubiquitinated AIF is a major mediator of hypoxia-induced mitochondrial dysfunction and pulmonary artery smooth muscle cell proliferation. Cell Biosci 12:9 (2022). PubMed: 35090552
    • Yehia L  et al. Non-canonical role of wild-type SEC23B in the cellular stress response pathway. Cell Death Dis 12:304 (2021). PubMed: 33753724
    • Wang F  et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res 11:7166-7185 (2019). PubMed: 31814919
    • Mao J  et al. Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52) is essential for preimplantation embryo development. Biol Open 7:N/A (2018). PubMed: 30135083
    • Li X  et al. Role of microRNA-4516 involved autophagy associated with exposure to fine particulate matter. Oncotarget 7:45385-45397 (2016). PubMed: 27329587
    • Kobayashi M  et al. The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development. Sci Rep 6:36780 (2016). PubMed: 27829658
    • Francis C  et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . PubMed: 25301173

    客户评价及客户问答

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    Question

    Dear madam/sir,

    I have a question concerning the Uba52 antibody, ab109227. The description mentions that this antibody was raised against a N-terminal peptide of the protein. However, because of the 100% similarity of the N-terminal half of Uba52 with Ubiquitin (ubb, ubc), is it to be expected that it will cross-react ? In other words, is the peptide it was raised against unique for Uba52 ?

    Kind regards,

    Read More
    Answer

    Thank you for your enquiry.

    I am sorry to confirm that the cross-reactivity of the ab109227 UBA52 antibody to other Ubiquitin proteins have not been verified by testing. However, based on sequence analysis, the immunogen does not share sequence identity with RPS27A, Ubiquitin B or Ubiquitin C.

    I am sorry we do not have more data to share with you on this occasion. However, I hope the information is helpful. If you have any further questions, please do not hesitate to contact me.

    Read More

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