Anti-UBA5抗体(ab177507)
Key features and details
- Rabbit polyclonal to UBA5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UBA5抗体
参阅全部 UBA5 一抗 -
描述
兔多克隆抗体to UBA5 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Cow, Chimpanzee, Gorilla
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免疫原
Synthetic peptide within Human UBA5 aa 325-375. The exact sequence is proprietary. (NP_079094.1)
Sequence:QEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITV AYTIP
Database link: Q9GZZ9 -
阳性对照
- Jurkat, 293T and HeLa whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 45 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 45 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
E1-like enzyme which activates UFM1 and SUMO2. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. -
细胞定位
Cytoplasm. Nucleus. Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2. - Information by UniProt
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数据库链接
- Entrez Gene: 460705 Chimpanzee
- Entrez Gene: 509292 Cow
- Entrez Gene: 101151272 Gorilla
- Entrez Gene: 79876 Human
- Omim: 610552 Human
- SwissProt: A7MAZ3 Cow
- SwissProt: Q9GZZ9 Human
- Unigene: 170737 Human
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别名
- 5730525G14Rik antibody
- AW240750 antibody
- FLJ23251 antibody
see all
图片
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Western blot - Anti-UBA5 antibody (ab177507)All lanes : Anti-UBA5 antibody (ab177507) at 0.4 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 45 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-UBA5 antibody (ab177507)Detection of UBA5 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177507 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab177507 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab177507 被引用在 2 文献中.
- Serrano RJ et al. Genetic model of UBA5 deficiency highlights the involvement of both peripheral and central nervous systems and identifies widespread mitochondrial abnormalities. Brain Commun 5:fcad317 (2023). PubMed: 38046095
- Schuren ABC et al. The UFM1 Pathway Impacts HCMV US2-Mediated Degradation of HLA Class I. Molecules 26:N/A (2021). PubMed: 33430125