AB124728

重组Anti-UBA3抗体[MILR42872]

Name: UBA3抗体[MILR42872] (ab124728)| Abcam中文官网
Brand: Abcam Limited
SKU: AB124728
Price: 2491 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-UBA3 antibody [MILR42872]

RabMAb
Recombinant
了解详情

(1 Review)

(12 Publications)

Rabbit Recombinant Monoclonal UBA3 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 12 publications.

查看别名

UBE1C, UBA3, NEDD8-activating enzyme E1 catalytic subunit, NEDD8-activating enzyme E1C, Ubiquitin-activating enzyme E1C, Ubiquitin-like modifier-activating enzyme 3, Ubiquitin-activating enzyme 3

1 Images

Unknown

Western blot - Anti-UBA3 antibody [MILR42872] (AB124728)

All lanes:

Western blot - Anti-UBA3 antibody [MILR42872] (ab124728)

Lane 1:

Fetal lung cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

HepG2 cell lysate at 10 µg

Lane 6:

K562 cell lysate at 10 µg

Predicted band size: 52 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-UBA3 antibody [MILR42872] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

MILR42872

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The target UBA3 also known as ubiquitin-like modifier-activating enzyme 3 functions mechanically as an essential enzyme in the NEDD8-conjugation pathway. It weighs approximately 50 kDa. UBA3 expresses itself in various tissues but shows widespread expression across different cell types. It operates as a catalytic subunit of the E1 enzyme complex that initiates the activation and transfer of the NEDD8 protein. This process is important for the subsequent conjugation to target proteins.

Biological function summary

The process involving UBA3 relates to protein modification primarily through neddylation. UBA3 couples with the protein NAE1 to form the NEDD8 activating enzyme complex. This enzyme complex activates NEDD8 a ubiquitin-like protein that modifies cullin-based E3 ligases influencing their function. By modifying these E3 ligases UBA3 controls various cellular processes including cell cycle progression and signal transduction ensuring proper cell function and adaptation to environmental cues.

Pathways

UBA3 is integral to the NEDD8 conjugation pathway influencing cell cycle and transcriptional regulation. It interacts with proteins such as cullin 1 and cullin 3 within this pathway. By activating NEDD8 UBA3 facilitates cullin-RING ubiquitin ligase complexes which play significant roles in the ubiquitin-proteasome pathway. This interaction controls protein degradation a vital process for maintaining protein homeostasis and preventing cellular dysfunction.

UBA3 involvement in neddylation links it to cancer and neurodegenerative diseases. Aberrant UBA3 activity relates to cancers as dysregulation of the NEDD8 pathway can lead to uncontrolled cell proliferation. In neurodegenerative disorders such as Alzheimer's disease altered neddylation can disrupt normal protein homeostasis contributing to neuron damage. UBA3's interaction with protein partners like cullins connects it to various pathological states highlighting its importance in disease development and progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

See full target information UBA3

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10536 PubMed39627198

2024

CRL3 E3 ligase facilitates ubiquitin-mediated degradation of oncogenic SRX to suppress colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhu,Liangshan Li,Yuanyuan Chen,Yongfu Pan,Wenjuan Zhang,Lihui Li,Lili Cai,Xiaoxue Zhao,Hu Zhao,Shiwen Wang,Lijun Jia

Nature communications 15:9820 PubMed39537588

2024

Succinate dehydrogenase deficiency-driven succinate accumulation induces drug resistance in acute myeloid leukemia via ubiquitin-cullin regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Chen,Miao Xian,Wenwen Ying,Jiayi Liu,Shaowei Bing,Xiaomin Wang,Jiayi Yu,Xiaojun Xu,Senfeng Xiang,Xuejing Shao,Ji Cao,Qiaojun He,Bo Yang,Meidan Ying

Cell stem cell 31:1162-1174.e8 PubMed38917806

2024

Genome-wide CRISPR screen identifies neddylation as a regulator of neuronal aging and AD neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Saurat,Andrew P Minotti,Maliha T Rahman,Trisha Sikder,Chao Zhang,Daniela Cornacchia,Johannes Jungverdorben,Gabriele Ciceri,Doron Betel,Lorenz Studer

Cell death & disease 13:844 PubMed36192389

2022

PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Shizhen Zhang,Xiahong You,Tiantian Xu,Qian Chen,Hua Li,Longyu Dou,Yilun Sun,Xiufang Xiong,Morgan A Meredith,Yi Sun

Nature communications 13:3034 PubMed35641493

2022

Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3 E3 ligase in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyin Zhou,Wenyu Lin,Chaoqun Wang,Fei Sun,Siwei Ju,Qian Chen,Yi Wang,Yongxia Chen,Haomin Li,Linbo Wang,Zeping Hu,Hongchuan Jin,Xian Wang,Yi Sun

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21800 PubMed34324733

2021

Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability.

Applications

Unspecified application

Species

Unspecified reactive species

Dacai Xu,Jiawen Wu,Jinghong Chen,Liling Jiang,Juan Chen,Wenhao Bao,Xin Chen,Qianqian Yang,Xiaolan Zhang,Leyi Yao,Huabo Su,Jinbao Liu

Neoplasia (New York, N.Y.) 22:179-191 PubMed32145688

2020

Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Yu,Zhiguo Hu,Yanwen Shen,Yihan Jiang,Peichen Pan,Tingjun Hou,Zhen-Qiang Pan,Jing Huang,Yi Sun

Oncology letters 18:2724-2732 PubMed31404297

2019

An overactive neddylation pathway serves as a therapeutic target and MLN4924 enhances the anticancer activity of cisplatin in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zeng,Yong-Shuang Iv,Qi-Hua Pan,Yi-Guo Zhou,He Li

JCI insight 4: PubMed30668548

2019

Inhibiting neddylation modification alters mitochondrial morphology and reprograms energy metabolism in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyin Zhou,Hua Li,Yuanyuan Li,Mingjia Tan,Shaohua Fan,Cong Cao,Feilong Meng,Ling Zhu,Lili Zhao,Min-Xin Guan,Hongchuan Jin,Yi Sun

eLife 6: PubMed28475037

2017

SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.

Applications

Unspecified application

Species

Unspecified reactive species

Kate E Coleman,Miklós Békés,Jessica R Chapman,Sarah B Crist,Mathew Jk Jones,Beatrix M Ueberheide,Tony T Huang

View all publications

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重组Anti-UBA3抗体[MILR42872]