AB174855

重组Anti-UAP1L1抗体[EPR11586]

Anti-UAP1L1 antibody [EPR11586]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal UAP1L1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

UDP-N-acetylhexosamine pyrophosphorylase-like protein 1, UAP1L1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-UAP1L1 antibody [EPR11586] (AB174855)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-UAP1L1 antibody [EPR11586] (AB174855)

Immunofluorescent staining of K562 cells labeling UAP1L1 using ab174855 at 1/50 dilution (red). DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-UAP1L1 antibody [EPR11586] (AB174855)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-UAP1L1 antibody [EPR11586] (AB174855)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling UAP1L1 using ab174855 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-UAP1L1 antibody [EPR11586] (AB174855)

All lanes:

Western blot - Anti-UAP1L1 antibody [EPR11586] (ab174855) at 1/1000 dilution

Lane 1:

Caco 2 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Predicted band size: 57 kDa

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关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11586

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The target UAP1L1 also known as UDP-N-acetylglucosamine pyrophosphorylase 2 or AGX2 is an enzyme with a molecular weight of approximately 63 kDa. This enzyme is responsible for catalyzing the conversion of UTP and N-acetylglucosamine-1-phosphate to UDP-N-acetylglucosamine an essential precursor for glycosylation processes. UAP1L1 expression occurs in various tissues including the liver and skeletal muscle but it is notably higher in testis tissue. Its activity impacts the synthesis of N-glycans and glycolipids important components of cellular membranes.

Biological function summary

UAP1L1 plays a significant role in cellular glycosylation. This process involves the addition of sugar moieties to proteins and lipids which is vital for proper folding stability and function of glycoproteins. UAP1L1 is not part of any known large enzyme complexes but it cooperates closely with other metabolic enzymes to maintain the pool of UDP-GlcNAc a high-energy substrate used in various biosynthetic pathways. It influences cellular homeostasis by regulating glycan biosynthesis.

Pathways

UAP1L1 is integral to the hexosamine biosynthetic pathway and the UDP-N-acetylglucosamine biosynthesis pathway. Through its enzymatic activity it contributes to maintaining cellular UDP-GlcNAc levels which correlate with key biosynthetic and signaling pathways including O-GlcNAcylation. UAP1L1 interacts with other enzymes like GFAT and OGT in these pathways highlighting its interconnected role in cellular metabolism growth and signaling.

Abnormal UAP1L1 function shows associations with metabolic and developmental disorders. Anomalies in hexosamine metabolism where UAP1L1 activity is important can lead to insulin resistance and contribute to the pathogenesis of type 2 diabetes. Alterations in glycosylation where UAP1L1 is a foundational player may also feature in congenital disorders of glycosylation. Proteins like OGT linked through pathways involving UAP1L1 dysregulation also relate to these diseases illustrating the interconnected nature of metabolic and glycosylation pathways in disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information UDP-N-acetylhexosamine pyrophosphorylase-like protein 1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

FASEB bioAdvances 7:e1479 PubMed39917395

2025

The human-specific noncoding RNA functions at the intersection of sexually dimorphic pathways in inflammation, senescence, and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Kameron Kennicott,Yun Liang

Molecular carcinogenesis 60:179-187 PubMed33434300

2021

Silencing of UAP1L1 inhibits proliferation and induces apoptosis in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiong Xiao,Lei Jiang,Huoli Hu,Yunhe Huang,Lun Yang,Yang Jiao,Guangxia Wei

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-UAP1L1抗体[EPR11586]

Anti-UAP1L1 antibody [EPR11586]

Immunocytochemistry/ Immunofluorescence - Anti-UAP1L1 antibody [EPR11586] (AB174855)

Flow Cytometry (Intracellular) - Anti-UAP1L1 antibody [EPR11586] (AB174855)

Western blot - Anti-UAP1L1 antibody [EPR11586] (AB174855)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原