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AB202140

Anti-U1A抗体

Anti-U1A antibody

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(1 Publication)

Rabbit Polyclonal U1A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNRPA.

查看别名

U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody (AB202140)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-U1A antibody (AB202140)

Immunofluorescent analysis of HeLa cells labeling U1A with ab202140 at 1/50 dilution.

Western blot - Anti-U1A antibody (AB202140)
  • WB

Supplier Data

Western blot - Anti-U1A antibody (AB202140)

All lanes:

Western blot - Anti-U1A antibody (ab202140) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

293T cell lysate

Lane 3:

A431 cell lysate

Lane 4:

MCF7 cell lysate

Lane 5:

Mouse brain tissue lysate

Lane 6:

Mouse testis tissue lysate

Lane 7:

Mouse thymus tissue lysate

Lane 8:

Rat brain tissue lysate

Predicted band size: 31 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF

applications

免疫原

Recombinant Full Length Protein corresponding to Human SNRPA.

P09012

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

U1A also known as snRNP U1A is a protein involved in the splicing of pre-messenger RNA. U1A is approximately 31 kDa in mass and functions as an important component of the U1 small nuclear ribonucleoprotein (snRNP) complex. Expressed in the cell nucleus U1A binds to the stem-loop structures of U1 RNA stabilizing the RNA-protein complex. This binding activity is critical as it allows U1 snRNP to recognize and bind splice sites on precursor mRNA facilitating the splicing process.
Biological function summary

U1A participates in the formation and function of the spliceosome a large RNA-protein complex essential for pre-mRNA processing. U1A as part of the U1 snRNP helps in recognizing and positioning the 5' splice site of introns in pre-mRNA. Through this role U1A ensures proper removal of non-coding sequences allowing for the maturation of mRNA. The spliceosome which includes other components like U2 U4 U5 and U6 snRNPs coordinates finely to execute the precise cutting and ligation of RNA sequences during splicing.

Pathways

U1A plays a central role in the mRNA splicing pathway which is a critical step in gene expression regulation. The splicing pathway interfaces with other biological pathways such as those involved in mRNA translation and degradation. U1A interacts with several proteins within these pathways notably SR proteins and hnRNPs which influence splice site selection and enhance the efficiency of splicing. These interactions highlight the interconnectedness of RNA processing events with overall gene expression controls.

U1A has been implicated in autoimmune diseases such as systemic lupus erythematosus (SLE). In SLE the body generates autoantibodies against several snRNP components including U1A which disrupts normal splicesome function. This leads to defective RNA processing and contributes to disease pathology. Additionally mutations or dysregulation of splicing proteins like SF3B1 and SRSF2 also associated with U1A through splicing abnormalities have been linked to various cancers where they lead to aberrant splicing patterns that drive tumorigenesis.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
See full target information SNRPA
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 51:3-13 PubMed31722080

2019

Natriuretic peptide system expression in murine and human submandibular salivary glands: a study of the spatial localisation of ANB, BNP, CNP and their receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Araz Ahmed,Alessandro Gulino,Simita Amayo,Walter Arancio,Ada Maria Florena,Beatrice Belmonte,Abdo Jurjus,Angelo Leone,Isabelle Miletich
View all publications
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