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AB6211

Anti-Tyrosine Hydroxylase抗体

Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

5

(4 Reviews)

|

(77 Publications)

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody detecting Tyrosine Hydroxylase in Western Blot, IHC-Fr, ICC/IF. Suitable for Mouse, Rat.

- Over 70 publications
- Trusted since 2000

查看别名

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • WB

Supplier Data

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

All lanes:

Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab6211) at 1/100 dilution

All lanes:

PC12 cell lysates at 6 µg

Predicted band size: 58 kDa

false

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • IHC-FoFr

Supplier Data

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1 : 100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)
  • IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (AB6211)

ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.

Image courtesy of QBM Cell Sciences, Ottawa, Canada

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat

应用

IHC-FoFr, ICC/IF, IHC-Fr, WB

applications

免疫原

Synthetic Peptide within Rat TH aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P04177

特异性

Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.

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反应性数据

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我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

Anti-Tyrosine Hydroxylase antibody (ab6211) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-FoFr, IHC-Fr, WB in mouse samples.

Anti-Tyrosine Hydroxylase antibody (ab6211) has been cited over 77 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Tyrosine Hydroxylase antibody (ab6211) has high sensitivity and specificity.

Anti-Tyrosine Hydroxylase antibody (ab6211) has 4 independent reviews from customers.

Anti-Tyrosine Hydroxylase antibody (ab6211) specifically detects Tyrosine Hydroxylase (UniProt ID: P04177; Molecular weight: 56kDa) and is sold in a 50 µL selling size.

This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
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性能和储存信息

形式
Liquid
纯度
Whole antiserum
存储溶液
pH: 7 - 7.4 Constituents: Whole serum
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Tyrosine hydroxylase also known as TH is an enzyme that catalyzes the conversion of L-tyrosine to L-DOPA. It weighs approximately 60 kDa. You can find this enzyme expressed mainly in the brain adrenal glands and some peripheral tissues. Commonly studied antibodies include anti-tyrosine hydroxylase or anti-TH. It plays a significant role in the catecholamine biosynthesis pathway serving as the rate-limiting step in dopamine synthesis.
Biological function summary

Tyrosine hydroxylase is essential for synthesizing catecholamines including dopamine norepinephrine and epinephrine. This enzyme associates with other components in the catecholamine biosynthetic pathway. It functions as part of a larger complex within certain cells where its action determines levels of important neurotransmitters. These neurotransmitters help regulate numerous central and peripheral nervous system activities.

Pathways

Tyrosine hydroxylase acts as a critical component in both the dopaminergic and adrenergic pathways. In the dopaminergic pathway tyrosine hydroxylase facilitates dopamine production influencing various physiological processes. The enzyme also correlates with ardrd protein in these pathways allowing fine-tuned control of neurotransmitter synthesis. Proteins related to tyrosine hydroxylase in these pathways include aromatic L-amino acid decarboxylase which further processes L-DOPA into dopamine.

Tyrosine hydroxylase has connections with neurodegenerative diseases like Parkinson's disease and conditions such as dystonia. Dysfunctional expression or activity of this enzyme can lead to dopamine deficiency contributing to the motor symptoms in Parkinson's disease. In dystonia alterations in tyrosine hydroxylase activity affect neurotransmitter balance influencing muscle coordination. The relationship with ardrd protein highlights tyrosine hydroxylase's role in maintaining neural health and it highlights potential areas for therapeutic intervention.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (PubMed : 30936473).
See full target information Th
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文献 (77)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7801 PubMed38016975

2023

Dbh catecholaminergic cardiomyocytes contribute to the structure and function of the cardiac conduction system in murine heart.

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Tianyi Sun,Alexander Grassam-Rowe,Zhaoli Pu,Yangpeng Li,Huiying Ren,Yanru An,Xinyu Guo,Wei Hu,Ying Liu,Yuqing Zheng,Zhu Liu,Kun Kou,Xianhong Ou,Tangting Chen,Xuehui Fan,Yangyang Liu,Shu Tu,Yu He,Yue Ren,Ao Chen,Zhouchun Shang,Zhidao Xia,Lucile Miquerol,Nicola Smart,Henggui Zhang,Xiaoqiu Tan,Weinian Shou,Ming Lei

Molecular neurobiology 61:3397-3408 PubMed37989982

2023

MiR-184 Mediated the Expression of ZNF865 in Exosome to Promote Procession in the PD Model.

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Chang Liu,Yang Wang,Jing-Wen Li,Xiaoyan Zhu,Hai-Song Jiang,Hong Zhao,Li-Ming Zhang

International journal of molecular sciences 24: PubMed37373475

2023

Low-Field Magnetic Stimulation Alleviates MPTP-Induced Alterations in Motor Function and Dopaminergic Neurons in Male Mice.

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Unspecified application

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Sathiya Sekar,Yanbo Zhang,Hajar Miranzadeh Mahabadi,Benson Buettner,Changiz Taghibiglou

Journal of neuroinflammation 20:117 PubMed37208728

2023

Dysbiosis of gut microbiota inhibits NMNAT2 to promote neurobehavioral deficits and oxidative stress response in the 6-OHDA-lesioned rat model of Parkinson's disease.

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Jianjun Yu,Jianhong Meng,Zhengwei Qin,Yuan Yu,Yingxin Liang,Yanjun Wang,Dongmei Min

International journal of molecular sciences 24: PubMed36835592

2023

The Biological Behaviors of Neural Stem Cell Affected by Microenvironment from Host Organotypic Brain Slices under Different Conditions.

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Qian Jiao,Li Wang,Zhichao Zhang,Xinlin Chen,Haixia Lu,Yong Liu

Translational psychiatry 13:17 PubMed36670104

2023

Fecal microbiota transplantation improves VPA-induced ASD mice by modulating the serotonergic and glutamatergic synapse signaling pathways.

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Jifeng Wang,Yuan Cao,Weiliang Hou,Dexi Bi,Fang Yin,Yaohui Gao,Dengfeng Huang,Yingying Li,Zhan Cao,Yinmei Yan,Jianhua Zhao,Dewu Kong,Xiaoqiong Lv,Linsheng Huang,Hui Zhong,Chunyan Wu,Qiyi Chen,Rong Yang,Qing Wei,Huanlong Qin

Frontiers in aging neuroscience 14:1020321 PubMed36248005

2022

Deep brain stimulation in the globus pallidus alleviates motor activity defects and abnormal electrical activities of the parafascicular nucleus in parkinsonian rats.

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Jinlu Xie,Zheng Chen,Tingting He,Hengya Zhu,Tingyu Chen,Chongbin Liu,Xuyan Fu,Hong Shen,Tao Li

Experimental and therapeutic medicine 24:635 PubMed36160902

2022

Effects of Brain Factor‑7® against motor deficit and oxidative stress in a mouse model of MPTP‑induced Parkinson's disease.

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Tae-Kyeong Lee,Jae-Chul Lee,Dae Won Kim,Ji-Won Lee,Sung-Su Kim,Hyung-Il Kim,Myoung Cheol Shin,Jun Hwi Cho,Moo-Ho Won,Soo Young Choi

Cancers 14: PubMed35740651

2022

Differential HIF2α Protein Expression in Human Carotid Body and Adrenal Medulla under Physiologic and Tumorigenic Conditions.

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Lucía Celada,Tamara Cubiella,Jaime San-Juan-Guardado,Andrés San José Martínez,Nuria Valdés,Paula Jiménez-Fonseca,Ignacio Díaz,Jose María Enguita,Aurora Astudillo,Enol Álvarez-González,Luisa María Sierra,María-Dolores Chiara

iScience 25:104388 PubMed35633939

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Dopamine modulates visual threat processing in the superior colliculus via D2 receptors.

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Quentin Montardy,Zheng Zhou,Lei Li,Qingning Yang,Zhuogui Lei,Xiaolong Feng,Shanping Chen,Qianqian Shi,Huiqi Zhang,Shuran Chen,Zhijian Zhang,Binghao Zhao,Fuqiang Xu,Zhonghua Lu,Liping Wang
View all publications
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