Anti-Tyrosine Hydroxylase抗体- Neuronal Marker (ab117112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Tyrosine Hydroxylase抗体- Neuronal Marker
参阅全部 Tyrosine Hydroxylase 一抗 -
描述
兔多克隆抗体to Tyrosine Hydroxylase - Neuronal Marker -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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免疫原
Synthetic peptide corresponding to Human Tyrosine Hydroxylase aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139516) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117112于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays an important role in the physiology of adrenergic neurons. -
组织特异性
Mainly expressed in the brain and adrenal glands. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
疾病相关
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
别名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab117112)IHC image of Tyrosine 3-monooxygenase staining in sagittal 6 week rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117112, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab117112 被引用在 10 文献中.
- Liu H et al. Renal nerve stimulation identifies renal innervation and optimizes the strategy for renal denervation in canine. J Transl Med 21:100 (2023). PubMed: 36759871
- Zhou L et al. N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity. Aging Cell 22:e13745 (2023). PubMed: 36437524
- Yu T et al. Cholestanol accelerates α-synuclein aggregation and spreading by activating asparagine endopeptidase. JCI Insight 8:N/A (2023). PubMed: 37937646
- Fikry H et al. Neuroprotective effects of curcumin on the cerebellum in a rotenone-induced Parkinson's Disease Model. CNS Neurosci Ther 28:732-748 (2022). PubMed: 35068069
- Chen LJ et al. Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus. Fluids Barriers CNS 19:95 (2022). PubMed: 36437472
- Rudak PT et al. Chronic stress physically spares but functionally impairs innate-like invariant T cells. Cell Rep 35:108979 (2021). PubMed: 33852855
- Irvine KA et al. Loss of diffuse noxious inhibitory control after traumatic brain injury in rats: A chronic issue. Exp Neurol 333:113428 (2020). PubMed: 32745472
- Du T et al. Injection of a-syn-98 Aggregates Into the Brain Triggers a-Synuclein Pathology and an Inflammatory Response. Front Mol Neurosci 12:189 (2019). PubMed: 31447645
- Chen WJ et al. The Impact of Renal Denervation on the Progression of Heart Failure in a Canine Model Induced by Right Ventricular Rapid Pacing. Front Physiol 10:1625 (2019). PubMed: 32082182
- Yun JW et al. Behavior, PET and histology in novel regimen of MPTP marmoset model of Parkinson's disease for long-term stem cell therapy. Tissue Eng Regen Med 13:100-109 (2016). PubMed: 30603390