Anti-Tyrosine Hydroxylase抗体- Neuronal Marker (ab112)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Tyrosine Hydroxylase抗体- Neuronal Marker
参阅全部 Tyrosine Hydroxylase 一抗 -
描述
兔多克隆抗体to Tyrosine Hydroxylase - Neuronal Marker -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Rat
预测可用于: Mammals
-
免疫原
Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase aa 1 to the C-terminus. Full length SDS denatured protein purified from rat pheochromocytoma.
Database link: P04177 -
阳性对照
- WB: PC-12 whole cell lysate. Rat caudate and striatal lysate. IHC-P: Rat brain tissue.
-
常规说明
ab112 can be used as a marker for dopaminergic and noradrenergic neurons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
靶标
-
功能
Plays an important role in the physiology of adrenergic neurons. -
组织特异性
Mainly expressed in the brain and adrenal glands. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
疾病相关
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
-
数据库链接
- Entrez Gene: 25085 Rat
- SwissProt: P04177 Rat
- Unigene: 11082 Rat
-
别名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
图片
-
Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).
Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrusab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.
The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an anonymous Abreviewab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.
The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.
-
Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg
Predicted band size: 60 kDa -
Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat striatal lysate at 10 µg
Predicted band size: 60 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (332)
ab112 被引用在 332 文献中.
- Dong AQ et al. Pramipexole inhibits astrocytic NLRP3 inflammasome activation via Drd3-dependent autophagy in a mouse model of Parkinson's disease. Acta Pharmacol Sin 44:32-43 (2023). PubMed: 35896696
- Ramírez-Plascencia OD et al. Leptin and adiponectin regulate the activity of nuclei involved in sleep-wake cycle in male rats. Front Neurosci 16:907508 (2022). PubMed: 35937866
- Bayley JP et al. Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas. PLoS One 17:e0274478 (2022). PubMed: 36178902
- Mazumder S et al. Post-mortem brain histological examination in the substantia nigra and subthalamic nucleus in Parkinson's disease following deep brain stimulation. Front Neurosci 16:948523 (2022). PubMed: 36188463
- Xu J et al. Therapeutic function of a novel rat induced pluripotent stem cell line in a 6‑OHDA‑induced rat model of Parkinson's disease. Int J Mol Med 50:N/A (2022). PubMed: 36300203