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Neuroscience Neurotransmitter Biogenic Amines Dopamine

Anti-Tyrosine Hydroxylase抗体- Neuronal Marker (ab112)

  • Datasheet
  • SDS
Reviews (36)Q&A (10)References (246)

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Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
  • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)

Key features and details

  • Rabbit polyclonal to Tyrosine Hydroxylase - Neuronal Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Rat
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Tyrosine Hydroxylase抗体- Neuronal Marker
    参阅全部 Tyrosine Hydroxylase 一抗
  • 描述

    兔多克隆抗体to Tyrosine Hydroxylase - Neuronal Marker
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Rat
    预测可用于: Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Rat Tyrosine Hydroxylase aa 1 to the C-terminus. Full length SDS denatured protein purified from rat pheochromocytoma.
    Database link: P04177

  • 阳性对照

    • WB: PC-12 whole cell lysate. Rat caudate and striatal lysate. IHC-P: Rat brain tissue.
  • 常规说明

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.50
    Constituents: 0.01% BSA, 0.87% Sodium chloride, 50% Glycerol, 0.238% HEPES
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Dopamine
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
  • Recombinant Protein

    • Recombinant Mouse Tyrosine Hydroxylase protein (His tag) (ab219278)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab112于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (16)
1/750.
WB (4)
1/200. Predicted molecular weight: 60 kDa.
说明
IHC-P
1/750.
WB
1/200. Predicted molecular weight: 60 kDa.

靶标

  • 功能

    Plays an important role in the physiology of adrenergic neurons.
  • 组织特异性

    Mainly expressed in the brain and adrenal glands.
  • 通路

    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 疾病相关

    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 序列相似性

    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Target information above from: UniProt accession P07101 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 25085 Rat
    • SwissProt: P04177 Rat
    • Unigene: 11082 Rat
    • 别名

      • Dystonia 14 antibody
      • DYT14 antibody
      • DYT5b antibody
      • EC 1.14.16.2 antibody
      • OTTHUMP00000011225 antibody
      • OTTHUMP00000011226 antibody
      • ple antibody
      • Protein Pale antibody
      • TH antibody
      • The antibody
      • TY3H_HUMAN antibody
      • TYH antibody
      • Tyrosine 3 hydroxylase antibody
      • Tyrosine 3 monooxygenase antibody
      • Tyrosine 3-hydroxylase antibody
      • Tyrosine 3-monooxygenase antibody
      • Tyrosine hydroxylase antibody
      see all

    图片

    • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)

      Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (Panel 1).

      Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an Abreview submitted by Dr Guillermo Estivill-Torrus

      ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P.

      The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)This image is courtesy of an anonymous Abreview

      ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry.

      The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

      See Abreview

    • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat caudate lysate at 10 µg

      Predicted band size: 60 kDa

    • Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112)
      Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab112) at 1/200 dilution + rat striatal lysate at 10 µg

      Predicted band size: 60 kDa

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (246)

    发表研究结果有使用 ab112?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab112 被引用在 246 文献中.

    • Liu Z  et al. Protective effect of thioredoxin reductase 1 in Parkinson's disease. Neurosci Lett 741:135457 (2021). PubMed: 33171211
    • Pasquettaz R  et al. Peculiar protrusions along tanycyte processes face diverse neural and nonneural cell types in the hypothalamic parenchyma. J Comp Neurol 529:553-575 (2021). PubMed: 32515035
    • Niklasson CU  et al. Hypoxia inducible factor-2a importance for migration, proliferation, and self-renewal of trunk neural crest cells. Dev Dyn 250:191-236 (2021). PubMed: 32940375
    • Shenton FC  et al. Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart. J Anat 238:36-52 (2021). PubMed: 32783212
    • Kern F  et al. Validation of human microRNA target pathways enables evaluation of target prediction tools. Nucleic Acids Res 49:127-144 (2021). PubMed: 33305319
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 46 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Nov 16 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

    Excellent
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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Midbrain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    Yes - .1% TritonX-100 in PBS
    Specification
    Midbrain
    Blocking step
    Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 May 17 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (embryonic)
    Permeabilization
    Yes - Tritonx100
    Specification
    embryonic
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jun 05 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Dopaminergic neuron)
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Dopaminergic neuron
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
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    Immunohistochemistry (Frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Sample
    Pig Tissue sections (Heart)
    Permeabilization
    Yes - Triton
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton 0.5%
    Specification
    brain
    Blocking step
    Serum as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Sample
    Rat Tissue sections (brainstem)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    brainstem
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
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    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Sep 07 2018

    Western blot abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Adipose fat)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    20 µg
    Specification
    Adipose fat
    Blocking step
    Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
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    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    Enzymatic
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
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    Western blot abreview for Anti-Tyrosine Hydroxylase antibody - Neuronal Marker

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    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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