重组Anti-Tyrosine Hydroxylase抗体[EP1532Y] –小鼠IgG1 (Chimeric) – BSA and Azide Free (ab317796)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317796)](https://www.abcam.cn/ps/products/317/ab317796/Images/ab317796-541572-anti-tyrosine-hydroxylase-antibody-ep1532y-mouse-igg1-chimeric-immunohistochemistry-brain-rat-new.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317796)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EP1532Y] to Tyrosine Hydroxylase - Chimeric – BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Rat
Related conjugates and formulations
概述
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产品名称
Anti-Tyrosine Hydroxylase抗体[EP1532Y] –小鼠IgG1 (Chimeric) – BSA and Azide Free
参阅全部 Tyrosine Hydroxylase 一抗 -
描述
小鼠单克隆抗体[EP1532Y] to Tyrosine Hydroxylase - Chimeric – BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Rat brain tissue.
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常规说明
AB317796 is the carrier-free version of AB317786.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab137869). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
EP1532Y -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab137869)
- HRP Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab207673)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - BSA and Azide free (ab220218)
- Alexa Fluor® 488 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab302827)
- Alexa Fluor® 568 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab302968)
- Alexa Fluor® 555 Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (ab305365)
- PE Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab310901)
- Alexa Fluor® 647 Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab311092)
- Alexa Fluor® 594 Anti-Tyrosine Hydroxylase antibody [EP1532Y] (ab311693)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Goat IgG (Chimeric) (ab317785)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Mouse IgG1 (Chimeric) (ab317786)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free (ab317795)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker – Chicken IgY (Chimeric) (ab318304)
- Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Chicken IgY (Chimeric) – BSA and Azide Free (ab318307)
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays an important role in the physiology of adrenergic neurons. -
组织特异性
Mainly expressed in the brain and adrenal glands. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
疾病相关
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 25085 Rat
- SwissProt: P04177 Rat
- Unigene: 11082 Rat
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别名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317796)
Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317796 at 1/500 dilution, and then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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![Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317796)](https://www.abcam.cn/ps/products/317/ab317796/Images/ab317796-542318-BenefitsofRecombinantantibodies.jpg)
Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317796)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab317796 尚未被引用在任何文献中。
