Anti-Tyrosine Hydroxylase抗体(ab76442)
Key features and details
- Chicken polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-P, IHC-FrFl
- Reacts with: Mouse
- Isotype: IgY
概述
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产品名称
Anti-Tyrosine Hydroxylase抗体
参阅全部 Tyrosine Hydroxylase 一抗 -
描述
鸡多克隆抗体to Tyrosine Hydroxylase -
宿主
Chicken -
经测试应用
适用于: IHC-P, IHC-FrFlmore details
不适用于: WB -
种属反应性
与反应: Mouse -
免疫原
Two synthetic peptide/ keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101) and mouse (P24529) sequences.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.02% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Two different affinity-purified anti-peptide antibodies were combined to make this product. After repeated injections of synthetic peptides into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 100 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized. The concentrations of both antibodies were 100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200 ug/ml. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76442于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (3) |
1/1000 - 1/2000.
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| IHC-FrFl | (3) |
1/1000.
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| 说明 |
|---|
|
IHC-P
1/1000 - 1/2000. |
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IHC-FrFl
1/1000. |
靶标
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功能
Plays an important role in the physiology of adrenergic neurons. -
组织特异性
Mainly expressed in the brain and adrenal glands. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
疾病相关
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 21823 Mouse
- SwissProt: P24529 Mouse
- Unigene: 1292 Mouse
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别名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
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Immunohistochemistry - Free Floating - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Free Floating) analysis of the rostral ventral lateral medulla in adult mouse brain labelling tyrosine hydroxylase with ab76442 at a dilution of 1/1000. Tissue was fixed with 10% formalin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab76442)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse hippocampus tissue labelling Tyrosine Hydroxylase (red) with ab76442. Tissue was fixed with 4% paraformaldehyde. Green: GFP autofluorescence (under control of actin promoter).
数据表及文件
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SDS download
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Datasheet download
文献 (160)
ab76442 被引用在 160 文献中.
- Tseng CT et al. Frequency Specific Optogenetic Stimulation of the Locus Coeruleus Induces Task-Relevant Plasticity in the Motor Cortex. J Neurosci 44:N/A (2024). PubMed: 38124020
- Lin G et al. Exploring therapeutic strategies for infantile neuronal axonal dystrophy (INAD/PARK14). Elife 12:N/A (2023). PubMed: 36645408
- Seo DC et al. DDC-Promoter-Driven Chemogenetic Activation of SNpc Dopaminergic Neurons Alleviates Parkinsonian Motor Symptoms. Int J Mol Sci 24:N/A (2023). PubMed: 36768816
- Parrales-Macias V et al. The Pesticide Chlordecone Promotes Parkinsonism-like Neurodegeneration with Tau Lesions in Midbrain Cultures and C. elegans Worms. Cells 12:N/A (2023). PubMed: 37174736
- Rájová J et al. Deconvolution of spatial sequencing provides accurate characterization of hESC-derived DA transplants in vivo. Mol Ther Methods Clin Dev 29:381-394 (2023). PubMed: 37251982