Anti-Tyrosine Hydroxylase抗体(ab137721)
Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Tyrosine Hydroxylase抗体
参阅全部 Tyrosine Hydroxylase 一抗 -
描述
兔多克隆抗体to Tyrosine Hydroxylase -
宿主
Rabbit -
经测试应用
适用于: IHC-FoFr, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 150-490 of Human Tyrosine Hydroxylase.
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阳性对照
- NT2D1, PC-3, U87-MG, SK-N-SH, Mouse brain and PC-12 whole cell lysates; SK-N-SH cells; Human H661 xenograft and Rat hindlimb pad skin tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-FoFr
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) before commencing with IHC staining protocol. |
靶标
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功能
Plays an important role in the physiology of adrenergic neurons. -
组织特异性
Mainly expressed in the brain and adrenal glands. -
通路
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. -
疾病相关
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. - Information by UniProt
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数据库链接
- Entrez Gene: 7054 Human
- Entrez Gene: 21823 Mouse
- Entrez Gene: 25085 Rat
- Omim: 191290 Human
- SwissProt: P07101 Human
- SwissProt: P24529 Mouse
- SwissProt: P04177 Rat
- Unigene: 435609 Human
see all -
别名
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraffin-embedded H661 xenograft labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
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Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/1000 dilution + PC-12 whole cell lysate at 30 µg
Predicted band size: 58 kDa
7.5% SDS-PAGE -
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)All lanes : Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution
Lane 1 : NT2D1 whole cell lysate
Lane 2 : PC-3 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : SK-N-SH whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5 % SDS-PAGE -
Western blot - Anti-Tyrosine Hydroxylase antibody (ab137721)Anti-Tyrosine Hydroxylase antibody (ab137721) at 1/500 dilution + Mouse brain lysate at 50 µg
Predicted band size: 58 kDa
7.5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunofluorescent analysis of methanol-fixed SK-N-SH cells labelling Tyrosine Hydroxylase with ab137721 at 1/500 dilution (left panel) and co-stained with Hoechst 33343 (right panel). SK-N-SH cells were fixed in -20°C 100% MeOH for 5 min. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab137721)Immunohistochemical analysis of paraformaldehyde-fixed frozen sections of Rat hindlimb pad skin tissue labelling Tyrosine Hydroxylase with ab137721 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab137721 被引用在 2 文献中.
- Pyon WS et al. An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue. Front Neuroanat 13:73 (2019). PubMed: 31379520
- Chiu HY et al. Long-lasting alterations in 5-HT2A receptor after a binge regimen of methamphetamine in mice. Int J Neuropsychopharmacol 17:1647-58 (2014). PubMed: 24763081