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Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-Tyrosinase抗体[T311] (ab738)

  • Datasheet
Reviews (2)Q&A (4)References (14)

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Key features and details

  • Mouse monoclonal [T311] to Tyrosinase
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

选择批间可重复性更高的重组抗体

Product image
Anti-Tyrosinase antibody [TYR/2024R] (ab236495)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Tyrosinase抗体[T311]
    参阅全部 Tyrosinase 一抗
  • 描述

    小鼠单克隆抗体[T311] to Tyrosinase
  • 宿主

    Mouse
  • 特异性

    Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Tyrosinase.

  • 阳性对照

    • Melanoma
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 20/07/17. The following lots are from ascites and are still in stock as of 20/07/17 – GR278593. Lot numbers higher than GR278593 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    T311
  • 骨髓瘤

    unknown
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • Tyrosinase Inhibitor Assay Kit (Colorimetric) (ab204715)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human Tyrosinase protein (Tagged) (ab152776)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab738于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/100.
说明
IHC-P
1/100.

靶标

  • 功能

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • 疾病相关

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • 序列相似性

    Belongs to the tyrosinase family.
  • 细胞定位

    Melanosome membrane.
  • Target information above from: UniProt accession P14679 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7299 Human
    • Omim: 606933 Human
    • SwissProt: P14679 Human
    • Unigene: 503555 Human
    • 别名

      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab738?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab738 被引用在 14 文献中.

    • Chen RH  et al. Dedifferentiation of human epidermal melanocytes in vitro by long-term trypsinization. Cell Tissue Bank 22:67-75 (2021). PubMed: 32978700
    • Jeong YM  et al. 3D-Printed Collagen Scaffolds Promote Maintenance of Cryopreserved Patients-Derived Melanoma Explants. Cells 10:N/A (2021). PubMed: 33800001
    • Du R & Lei T Effects of autologous platelet-rich plasma injections on facial skin rejuvenation. Exp Ther Med 19:3024-3030 (2020). PubMed: 32256789
    • Maitituoheti M  et al. Enhancer Reprogramming Confers Dependence on Glycolysis and IGF Signaling in KMT2D Mutant Melanoma. Cell Rep 33:108293 (2020). PubMed: 33086062
    • Kawakami T  et al. Approach for the Derivation of Melanocytes from Induced Pluripotent Stem Cells. J Invest Dermatol 138:150-158 (2018). PubMed: 28887108
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A375 - melanoma cell line)
    Loading amount
    10000 cells
    Specification
    A375 - melanoma cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 31 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    提交于 Sep 06 2011

    Question

    What is the concentration of lot GR33380?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2012

    Answer

    Thank you for contacting us.

    The IgG concentration of lot GR33380 is 66.7ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jul 17 2012

    Question

    I would like to enquire about recombinant controls for antibodies I recently purchased from you. Are there any recombinant controls or alternative controls for western blotting for the following antibodies; ab738 ab22601 ab1872 ab23798

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2005

    Answer

    Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab9788. Also, the antibody clearly stains IL1b positive cells in liver, spleen, pituitary and brain of endotoxin treated rats, so those tissues can be used as a positive control as well. Ab1872 - Placenta is recommended as a positive control. Also, caspase 1 is expressed in larger amounts in spleen and lung. Also found in liver, heart, skeletal muscle, kidney and testis. No expression in the brain. Ab738 - Melanoma is recommended as a positive control. Ab22601 - I couldn't find much information except for this: Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin. The gene expression is frequently activated in carcinoma derived cell lines.

    Read More

    Abcam Scientific Support

    回复于 Nov 15 2005

    Question

    Has the epitope been mapped?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2005

    Answer

    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

    Read More

    Abcam Scientific Support

    回复于 Mar 11 2005

    Question

    Your spec-sheet for tyrosinase antibody (ab738) does not have any information for western blotting. I would like to know at what size the band comes out, and how much I need to dilute the antibody for western blotting. And what kind of western protocol would you recommend me using, milk based? BSA-based? Or other ? I would appreciate your prompt response.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2005

    Answer

    Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminary experiment to determine the optimal dilution (a range of dilutions should be tried e.g 1:50-1:500) and we usually recommend to try BSA 5% in TBST (1hr, RT or 4C) for blocking. Please do not hesitate to contact us if you need any more advice or information or have any problems with your experiment,

    Read More

    Abcam Scientific Support

    回复于 Feb 27 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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