重组Anti-Tyrosinase抗体[EPR10141] (ab170905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10141] to Tyrosinase
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tyrosinase抗体[EPR10141]
参阅全部 Tyrosinase 一抗 -
描述
兔单克隆抗体[EPR10141] to Tyrosinase -
宿主
Rabbit -
特异性
This antibody does not react with mouse species in Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) application.
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经测试应用
适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human Tyrosinase. The exact sequence is proprietary.
Database link: P14679 -
阳性对照
- WB: Human melanoma lysate, SK-MEL-28. MeWo, B16-F0 and B16-F10 whole cell lysates. IHC-P: Human melanoma and skin tissue. ICC/IF: A375 cells. Flow Cyt (Intra): A375 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Constituents: 0.35% Sodium citrate, 0.17% Sodium chloride, 0.03% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10141 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170905于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
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ICC/IF |
1/50 - 1/100.
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Flow Cyt (Intra) |
1/30.
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说明 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Predicted molecular weight: 60 kDa. |
ICC/IF
1/50 - 1/100. |
Flow Cyt (Intra)
1/30. |
靶标
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功能
This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. -
疾病相关
Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. -
序列相似性
Belongs to the tyrosinase family. -
细胞定位
Melanosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7299 Human
- Entrez Gene: 22173 Mouse
- Omim: 606933 Human
- SwissProt: P14679 Human
- SwissProt: P11344 Mouse
- Unigene: 503555 Human
- Unigene: 238127 Mouse
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别名
- ATN antibody
- CMM8 antibody
- LB24 AB antibody
see all
图片
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All lanes : Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution
Lane 1 : B16-F0 (mouse melanoma epithelial-like cell), whole cell lysate
Lane 2 : B16-F10 (mouse melanoma epithelial-like cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 50-80 kDa why is the actual band size different from the predicted?
Exposure time: 59 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBSTThe expression profile observed is consistent with what has been described in the literature (PMID: 20053998; PMID: 24933607).
Negative control: NIH/3T3, RAW264.7 whole cell lysates. -
Immunohistochemical analysis of paraffin-embedded human skin tissue labelling Tyrosinase with ab170905 at 1/500 (0.662 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human skin. The section was incubated with ab170905 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human melanoma tissue, labeling Tyrosinase using ab170905 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab170905 staining Tyrosinase in A375 (human malignant melanoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only.
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All lanes : Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution
Lane 1 : SK-MEL-28 (Human malignant melanoma) whole cell lysate
Lane 2 : MeWo (Human malignant melanoma fibroblast) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST.
Exposure:
Lane 1: 20 seconds
Lane 2: 60 seconds
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Anti-Tyrosinase antibody [EPR10141] (ab170905) at 1/1000 dilution + Human melanoma lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa -
Intracellular Flow Cytometry analysis of A375 (human malignant melanoma) cells labeling with purified ab170905 at 1/30 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab170905 被引用在 20 文献中.
- Ferreira Branquinho MS et al. Kynurenine inhibits melanogenesis in human melanocyte-keratinocyte co-cultures and in a reconstructed 3D skin model. Exp Dermatol 31:427-432 (2022). PubMed: 34710259
- Hu SH et al. sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes. Oxid Med Cell Longev 2022:1856830 (2022). PubMed: 35186181
- Wu X et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res 35:328-341 (2022). PubMed: 35218147
- Liu Y et al. Ablation of Proton/Glucose Exporter SLC45A2 Enhances Melanosomal Glycolysis to Inhibit Melanin Biosynthesis and Promote Melanoma Metastasis. J Invest Dermatol 142:2744-2755.e9 (2022). PubMed: 35469906
- Kamo H et al. Tyrosinase suppresses vasculogenic mimicry in human melanoma cells. Oncol Lett 23:169 (2022). PubMed: 35496574