Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-TYRO3抗体[EPR4308] (ab109231)

Submit a review Submit a question References (14)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
  • Anti-TYRO3 antibody [EPR4308] (ab109231)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4308] to TYRO3
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-TYRO3抗体[EPR4308]
    参阅全部 TYRO3 一抗

  • 描述

    兔单克隆抗体[EPR4308] to TYRO3

  • 宿主

    Rabbit

  • 特异性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Fetal brain, fetal hippocampus, MCF7, Rat hippocampus, Rat brain, Human fetal brain, and Mouse hippocampus lysates IP: SH-SY5Y whole cell lysate IHC-P: Human brain tissue, Human colon carcinoma and Human ovarian carcinoma

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109231于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
IP
1/200.

For unpurified use at 1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
IP
1/200.

For unpurified use at 1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.

  • 组织特异性

    Abundant in the brain and lower levels in other tissues.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Brt antibody
    • BYK antibody
    • DTK antibody
    • Etk-2 antibody
    • protein tyrosine kinase 3 antibody
    • Rek antibody
    • RSE antibody
    • SKY antibody
    • Tif antibody
    • tyro3 antibody
    • TYRO3 protein tyrosine kinase antibody
    • TYRO3_HUMAN antibody
    • Tyrosine-protein kinase byk antibody
    • Tyrosine-protein kinase DTK antibody
    • Tyrosine-protein kinase receptor TYRO3 antibody
    • Tyrosine-protein kinase RSE antibody
    • Tyrosine-protein kinase SKY antibody
    • Tyrosine-protein kinase TIF antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified)

    Lane 1 : Rat hippocampus lysates at 20 µg
    Lane 2 : Rat brain lysates at 20 µg
    Lane 3 : Human fetal brain lysates at 15 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 97 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution (Purified) + Mouse hippocampus lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Immunoprecipitation - Anti-TYRO3 antibody [EPR4308] (ab109231)

    ab109231 (purified) at 1:200 dilution (2µg) immunoprecipitating TYRO3 in SH-SY5Y whole cell lysate.
    Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109231 & SH-SY5Y whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109231 in SH-SY5Y whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:2000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling TYRO3 with purified ab109231 at 1:500 dilution (7.44 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TYRO3 antibody [EPR4308] (ab109231)

    Immunohistochemical analysis of TYRO3 in paraffin embedded Human brain tissue, using ab109231 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    Western blot - Anti-TYRO3 antibody [EPR4308] (ab109231)
    All lanes : Anti-TYRO3 antibody [EPR4308] (ab109231) at 1/1000 dilution

    Lane 1 : fetal brain lysates
    Lane 2 : fetal hippocampus lysates
    Lane 3 : MCF7 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 97 kDa

  • Anti-TYRO3 antibody [EPR4308] (ab109231)
    Anti-TYRO3 antibody [EPR4308] (ab109231)

文献 (14)

发表研究结果有使用 ab109231？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109231 被引用在 14 文献中.

  • Zhong F  et al. TYRO3 agonist as therapy for glomerular disease. JCI Insight 8:N/A (2023). PubMed: 36454644
  • Vago JP  et al. Gas6/Axl Axis Activation Dampens the Inflammatory Response in Osteoarthritic Fibroblast-like Synoviocytes and Synovial Explants. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37242486
  • Wang J  et al. Overexpression of TYRO3 indicates poor prognosis and induces gastric cancer progression via AKT-mTOR pathway. Mol Carcinog 62:1325-1337 (2023). PubMed: 37212497
  • Wang J  et al. A cathelicidin antimicrobial peptide from Hydrophis cyanocinctus inhibits Zika virus infection by downregulating expression of a viral entry factor. J Biol Chem 298:102471 (2022). PubMed: 36089062
  • Vullings J  et al. Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis. J Immunol Res 2020:9690832 (2020). PubMed: 32964059
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

There are currently no Customer reviews or Questions for ab109231.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com