重组Anti-TXNRD2抗体[EPR12480] (ab180493)
![Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)](https://www.abcam.cn/ps/products/180/ab180493/Images/ab180493-410315-anti-txnrd2-antibody-epr12480-western-blot-human-knockout-lysate.jpg "Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12480] to TXNRD2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TXNRD2抗体[EPR12480]
参阅全部 TXNRD2 一抗 -
描述
兔单克隆抗体[EPR12480] to TXNRD2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293T, LnCap, A431 and HeLa whole cell lysate (ab150035); fetal liver and heart lysates. IHC-P: Human liver tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12480 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180493于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling. -
组织特异性
Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low. -
序列相似性
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 10587 Human
- Entrez Gene: 26462 Mouse
- Entrez Gene: 50551 Rat
- Omim: 606448 Human
- SwissProt: Q9NNW7 Human
- SwissProt: Q9JLT4 Mouse
- SwissProt: Q9Z0J5 Rat
- Unigene: 443430 Human
see all -
别名
- mitochondrial antibody
- Selenoprotein Z antibody
- SelZ antibody
see all
图片
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Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)All lanes : Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : TXNRD2 knockout HEK293T cell lysate
Lane 3 : LNCap cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDaLanes 1-4: Merged signal (red and green). Green - ab180493 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.
ab180493 Anti-TXNRD2 antibody [EPR12480] was shown to specifically react with TXNRD2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267267 (knockout cell lysate ab258259) was used. Wild-type and TXNRD2 knockout samples were subjected to SDS-PAGE. ab180493 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] (ab180493)](https://www.abcam.cn/ps/products/180/ab180493/Images/ab180493-211955-anti-txnrd2-antibody-epr12480-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD2 antibody [EPR12480] (ab180493)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TXNRD2 with ab180493 at 1/500 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)](https://www.abcam.cn/ps/products/180/ab180493/Images/ab180493-211953-anti-txnrd2-antibody-epr12480-western-blot.jpg)
Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)All lanes : Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/5000 dilution
Lane 1 : Fetal liver tissue lysate
Lane 2 : Fetal heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)](https://www.abcam.cn/ps/products/180/ab180493/Images/ab180493-211954-anti-txnrd2-antibody-epr12480-western-blot.jpg)
Western blot - Anti-TXNRD2 antibody [EPR12480] (ab180493)All lanes : Anti-TXNRD2 antibody [EPR12480] (ab180493) at 1/1000 dilution
Lane 1 : LnCap cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugate at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Anti-TXNRD2 antibody [EPR12480] (ab180493)](https://www.abcam.cn/ps/products/180/ab180493/Images/ab180493-5-benefits-of-recombinant-antibodies.png)
Anti-TXNRD2 antibody [EPR12480] (ab180493)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (9)
ab180493 被引用在 9 文献中.
- Jankowski CSR & Rabinowitz JD Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate. Cancer Res 82:3486-3498 (2022). PubMed: 35916672
- Evke S et al. Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen. Genes (Basel) 13:N/A (2022). PubMed: 35327975
- Schwarz M et al. Copper interferes with selenoprotein synthesis and activity. Redox Biol 37:101746 (2020). PubMed: 33059313
- Leonardi A et al. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene. Epigenetics 15:1121-1138 (2020). PubMed: 32303148
- Gao J et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
- Tarpey MD et al. Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness. J Physiol 597:869-887 (2019). PubMed: 30556208
- Gao J et al. TrxR2 overexpression alleviates inflammation-mediated neuronal death via reducing the oxidative stress and activating the Akt-Parkin pathway. Toxicol Res (Camb) 8:641-653 (2019). PubMed: 31588341
- Wang H et al. AMPKa2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med 121:202-214 (2018). PubMed: 29753072
- Blecha J et al. Antioxidant defense in quiescent cells determines selectivity of electron transport chain inhibition-induced cell death. Free Radic Biol Med 112:253-266 (2017). PubMed: 28774815